Radioligand binding assays, use

The results of this saturation assay were validated by direct comparison to conventionally conducted radioligand binding assays using [ H]NO 711 as marker [80, 100]. Not only due to financial considerations, hot/cold dilutions had to be used in the radioligand binding assays, in contrast to the MS binding assays... 

ASSOCIATION CONSTANTS AND SITE DENSITIES CALCULATED FROM COMPETITIVE RADIOLIGAND BINDING ASSAYS USING MIPs... 

There should be specific, saturable binding to the receptor, accompanied by pharmacological characteristics appropriate to the functional effects, demonstrable using a radioactive, eg, tritium or iodine-125, ligand to label the receptor. Radioligand binding assays (1,6) have become a significant means by which to identify and characterize receptors and enzymes (see Immunoassays Radioactive tracers). Isolation of the receptor or expression of the receptor in another cell, eg, an oocyte can be used to confirm the existence of a discrete entity. 

Radioligand binding assays are a relatively simple but at the same time a very important and efficient tool to study target-ligand interactions. Since they are very videly used in drug discovery and have been described extensively, this section only discusses their fundamental aspects and those aspects that are important for the description of the MS binding assays below . More detailed information can be found in the relevant literature [6, 7, 16-21]. 

Wang, J.-X., Yamamura, H. I., Wang, W, Roeske, W. R. The use of the filtration technique in in vitro radioligand binding assays for membrane-bound and solubilized receptors, in Receptor-Ligand Interactions. A Practical Approach, ed. Hulme, E. C., IRL Press, Oxford,... 

Although a very reliable radioligand binding assay with use The search for P2X receptor of a stable ATP analog (e.g. a, p-methylene ATP) and subtype selective ligands... 

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