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Radio TLC

Table 6 Summary of the C detection limits of various radio TLC methods [77]... Table 6 Summary of the C detection limits of various radio TLC methods [77]...
Using this approach, 2-[18F]-FDG 254 was obtained in 38% radiochemical yield, with a purity of 97.6% (determined by radio TLC). Miniaturization of the process enabled the reaction sequence to be performed in... [Pg.185]

Thin-layer radiochromatography (radio-TLC) is widely applied for a variety of environmental studies involving radiolabeled pesticides, such as plant uptake from soil, bioaccumulation in fish, dissipation from soil, metabolism in soil, plants, and fish, and environmental fate. The determination of the lipophiUdty of pesticides is important because their bioaccumulation and tendency for degradation and biotransformation are related to lipid solubility. TLC has advantages for lipohilicity studies compared to traditional partition coefficient measurement in an octanol-water system. [Pg.1151]

Such phenomena dictated two major philosophies for our work with pyrethroids (a) a mass balance approach was taken to account for all losses in the experiments (b) as long as the pyrethroids maintained their chemical integrity (did not hydrolyse or otherwise degrade) then use of radiolabelled analytes was desirable. This allowed the low concentrations of the pyrethroids in the aqueous phase to be accurately and reproducibly determined and the mass balance to be computed. The chemical integrity of the radiochemicals was determined by radio-chromatographic techniques (radio-TLC and radio-HPLC). [Pg.178]

Radioactive isotopes have been used since the mid-1940s for the elucidation of metabolic irregularities. Today, radionuchdes - principally H, and I - are used as tracers in all areas of pharmaceutical and biological research and in genetic technology [107]. In classical radio TLC, from the early 1960s, labeled prepared samples were applied to the TLC plate, which was then developed, and the p radiation was then measured with a TLC scaimer (raytest). [Pg.144]

Today, in radio TLC the radioactive-labeled molecules are transported by the migrating solvent along the thin-layer trace and are finally stay at a certain point along the trace on the TLC. The radioactive fraction is distributed equally in the depth of the thin-layer material (silica gel) between the surface and bottom. [Pg.144]

Further information on radio TLC can be obtained from the company raytest (see Section 12.5). [Pg.144]

The original version of this form (see p. 213) was produced in 1988 in accordance with the present author s instructions and was pubhshed in 1992 at a Merck Symposium [154]. Over the years, it has been modified to comply with more stringent requirements. For example, the parameters relative humidity (following a recommendation of Nyiredy [26]) and other evaluations (which could refer, e.g., to video scan or radio TLC) were included for the first time in 1997. To give the user an example of a universal type of form for TLC, the heading is given here in its original version, and the explanations below are listed. [Pg.216]

Figure 2. Radio-TLC- analysis of components extracted by 20% aqueous MeOH from foliage of susceptible "Rothamsted" and resistant "Peldon" black-grass immediately after 24h treatment with C1 -chlorotoluron. Eluent 20% EtOH/CHCl3. Figure 2. Radio-TLC- analysis of components extracted by 20% aqueous MeOH from foliage of susceptible "Rothamsted" and resistant "Peldon" black-grass immediately after 24h treatment with C1 -chlorotoluron. Eluent 20% EtOH/CHCl3.
Radio TLC profiles of brassinosteroid glycosylation in cell cultures of Lycopersicum esculentunr. [Pg.526]

Thin-layer radiochromatography (radio-TLC) is widely applied for a variety of environmental studies involving... [Pg.1751]

Fujino et al. described an interesting radio-TLC method for the analysis of paclitaxel and its 3-OH and 6-OH metabolites. After incubation of " C-paclitaxel with human liver microsomes, the supernatants were developed using a solvent system consisting of toluene-acetone-formic acid (60 39 1, v/v) and quantified with a bioimaging analyzer. The results obtained correlated well with those from RP-HPLC. [Pg.2289]

Gattavecchia, E., Tonelli, D., Breccia, A., Fini, A., and Ferri, E. (1994). Radio TLC in quality control and development of radiopharmaceuticals. J. Radioanal. Nucl. Chem. 181 77-84. [Pg.264]

Radio-TLC techniques are de.scribed in Chapter 12 of this Handbook, and in Chapter 13 of Ref. land Ref. 209-211. [Pg.39]

Another major step forward for radio-TLC came in the early 1980s when the so-called linear analyzer was introduced. This instrument was easier to use and more sensitive than the old scanners and was automated to the extent that up to four plates could be run overnight. More details are given below. As a consequence, improved quantitative results were obtained and analysis time was shortened. However, resolution was still not as good as that obtained by using autoradiography, and two-dimensional plates could not be easily evaluated. [Pg.349]

Pig. 6.1 Elongation studies on cis-AI modules, a SDS-PAGE gel of module X-TE labelled with 0 N-acteylcysteamine substrate and subsequent radio image of module X-TE labelling. KS inhibitor, cerulenin, clearly prevents acylation, b Radio-TLC for in vitro elongation assay conducted in the presence of NADPH. c Radio-TLC of in vitro elongation assay in the absence of NADPH. Adapted from [5]... [Pg.133]


See other pages where Radio TLC is mentioned: [Pg.226]    [Pg.197]    [Pg.201]    [Pg.202]    [Pg.202]    [Pg.270]    [Pg.524]    [Pg.527]    [Pg.986]    [Pg.2319]    [Pg.249]    [Pg.458]    [Pg.350]    [Pg.353]    [Pg.44]    [Pg.350]    [Pg.353]   
See also in sourсe #XX -- [ Pg.144 ]




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