Rabbits, fluorescent

In recent studies on perfused rats hearts (Veitch et al., 1992), it was found that differences in the sensitivity of complexes 1-lV to ischaemic damage were dependent upon the duration of ischaemia and the presence of oxygen. The demonstration that complex 1 is a major defective site dependent upon isolation of mitochondria from homogenates of the tissue by in vitro methods seemed important to us. We therefore decided to attempt to make noninvasive measurements of mitochondrial function soon after reperfusion in transplanted rabbit kidneys by surface fluorescence (for mitochondrial NADH levels) and near infra-red spectroscopy (NIRS) for the redox state of cytaas. 

Secondary antibody and determination. A secondary antibody labeled with an enzyme is added which binds to the primary antibody that is bound to the coating antigen. If the primary antibody were produced in a rabbit, an appropriate secondary antibody would be goat anti-rabbit immunoglobulin G (IgG) conjugated with horseradish peroxidase (HRP) (or another enzyme label). Excess secondary antibody is washed away. An appropriate substrate solution is added that will produce a colored or fluorescent product after enzymatic conversion. The amount of enzyme product formed is directly proportional to the amount of first antibody bound to the coating antigen on the plate and is inversely proportional to the amount of analyte in the standards. 

Elimination from the vitreous occurs by one of two pathways. This can be visualized by injecting fluorescent compounds and examining the concentration distribution in frozen sections obtained after a steady state has been established [230]. If the major route of elimination is by means of the re-tina/choroid, at steady state the lowest concentration would be in the vicinity of the retina. The contours observed in frozen sections of the rabbit eye obtained after intravitreal injection of fluorescein exhibit this pattern, with the highest concentration immediately behind the lens (Fig. 16A). Compounds not chiefly eliminated through the retina exit the vitreous by passive diffusion and enter the posterior aqueous, where they are eliminated by the natural production and outflow of aqueous humor. In such a situation, the contours would be perpendicular to the retina, with the highest concentration towards the rear of the vitreous cavity. This appears to be the case for fluorescently labeled dextran polymer, whose contours decrease in concentration toward the hyaloid membrane (Fig. 16B). 

Fig. 16 Contours of fluorescent intensity in frozen sections of the rabbit eye following 15 pL injection of marker solution in the central vitreous cavity injection was conducted through the superior rectus muscle 15 hours following injection of 0.2% sodium fluorescein 14 days following injection of 0.1% FITC-dextran, molecular weight 66,000. (From Ref. 230.)... 

Once internalized within the RPE, there must be a mechanism for carotenoid transport to photoreceptors. The RPE metabolizes lipids from phagocytosed POS and provides a constant supply of lipids to photoreceptors for the synthesis of new discs and molecular renewal of lipids within existing discs (Strauss, 2005). Thus there is a constant transfer of lipids from the RPE to photoreceptors. It has been shown in the rabbit and monkey that intraveneous administration of lipophilic benzopor-phyrin bound to LDLs results in an efficient delivery of the fluorescent photosensitizer not only to the RPE but also to photoreceptors this occurs within 20 min following injection (Haimovici et al., 1997 Miller et al., 1995). 

Wang S, Wang X, Shi W et al (2008) Detection of local polarity and conformational changes at the active site of rabbit muscle creatine kinase with a new arginine-specific fluorescent probe. Biochim Biophys Acta 1784 415 -22... 

Mannheimia haemo-lytica A1 leukotoxin 50 fused to green fluorescent protein White clover leaf Rabbits produced antibodies that recognized and neutralized leukotoxin. Immunogenic in rabbits after drying when delivered parenterally. 65... 

Rao et al.20 demonstrated a fluorescence polarization immunoassay for evaluating serum concentrations of tricyclic antidepressants (amitriptyline, imipramine, clomipramine, and doxepin) with respect to nonresponse, compliance, therapeutic window, and influences of age, sex, substance abuse, and toxicity. Abbott Laboratories TDx/TDxFLx Toxicology Tricyclic Assay FPIA (fluorescence polarization immunoassay) was used. This assay of 50 /uL samples contained tricyclic antidepressant antibodies raised in rabbits and fluorescein-labeled tricyclic antidepressant as a tracer. The assay was calibrated with imipramine in the range of 75 to 1000 fig/L (268 to 3571 nmol/L). Intra-assay and inter-assay coefficients of variation for internal quality control samples from the manufacturer were 4.2 and 4.7%, respectively. The limits of detection were 72,71,64, and 72 nmol/L for amitriptyline, imipramine, clomipramine, and doxepin, respectively. This high-throughput immunoassay was easy to use although amitriptyline, dosulepine, desipramine, and nortriptyline showed cross-reactivities ranging from 74 to 100%. 

R. Krapf, C. A. Berry, and A. S. Verkman, Estimation of intracellular chloride activity in isolated perfused rabbit proximal convoluted tubules using a fluorescent indicator, Biophys. J. 53, 955-962 (1988). 

A protein of similar molecular weight to that of rat oncomodulin, rat and rabbit parvalbumins, S100, and the vitamin D-dependent calcium-binding proteins has been isolated from chicken gizzard smooth muscle. In this case, however, the fluorescence emission from the four tyrosine residues is quenched by Ca2+ binding.(160) The decrease in fluorescence intensity was used to suggest that there are two different classes of Ca2+binding sites. 

A. Orstan, M. F. Lulka, B. Eide, P. H. Petra, and J. B. A. Ross, The steroid-binding site of human and rabbit sex steroid-binding protein of plasma Fluorescence characterization with... 

K. P. Kohse and L. M. Heilmeyer, The effects of Mg2+ on the Ca2+-binding properties and Ca2 +-induced tyrosine-fluorescence changes of calmodulin isolated from rabbit skeletal muscle, Eur. J. Biochem. 117, 507-513 (1981). 

Among multitryptophan proteins emitting light around 330 nm, we have observed the largest red-edge effect (estimated from the difference between the maxima of the fluorescence spectra obtained at 290- and 305-nm excitation) for papain in the active and inactive forms (13 and 10 nm, respectively). Large shifts were also observed for rabbit muscle asparagyl- and valyl-RNA synthetases (8 nm). For rabbit aldolase A, the observed shift was 6 nm, for skeletal muscle myosin, 4.5 nm, for chymotrypsin, 2.5 nm, and for carbonic... 

Figure 6.1. Fluorescence polarization immunoassay for theophylline. (A) Effect of theophylline rabbit antiserum ( ) and normal rabbit serum (A) on the fluorescence polarization of the theophylline-umbelliferone conjugate. (B) Fluorescence polarization of the theophylline-umbelliferone conjugate in the presence of varying concentrations of theophylline. (Reprinted from Ref. 1, with permission from Academic Press.)...

Stack and Gray have described a convenient, continuously recording, fluorescent assay for rabbit collagenase and gelatinase based on the hydroly-... 

Invasive continuous hepatic function monitoring by the fluorescence procedure was also evaluated in rabbits [148]. In this study, a commercial catheter equipped with fiber optic technology for mixed venous oxygen saturation measurements (SVO2) was modified to emit light at 780 nm and detect fluorescence at 840 nm. The catheter was placed into the right jugular vein and advanced... 

Fig. 11. Non-invasive in vivo fluorescence time-dependent clearance of ICG from plasma in a rabbit with normal functioning liver. The solid line is a single exponential fit to the measured data...

Fig. 5. (a) Layout of a slide that was spotted and immobilized using the anti goat IgG rabbit antibody and the anti rabbit IgG goat-antibody, (b) Fluorescent image after incubation of a Cy5-labeled goat IgG. (c) Fluorescent image after incubation of a Cy5-labeled rabbit IgG. 

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