Sodium fluorescein

The following are amongst the reagents that have been reported as being added to the mobile phase acids for quinine alkaloids [184], ninhydnn for amino acids [185 — 187], fluorescamine for biogenic amines [188] Fluorescein sodium [189], dichlorofluorescein [190], rhodamine 6G [191], ANS reagent [192] and bromine [193] have all been descnbed as additives to mobile phases... 

Note The full fluorescence intensity usually only develops about 30 min after the dipping process it then remains stable for several days if the chromatograms are stored in the dark (1, 5]. Fluorescein sodium can be employed in the reagent in place of 2, 7 -dichlorofluorescein [5]. The detection limits lie in the lower nanogram to picogram range [1, 5]. 

Augentropfen (Thilo) fluorescein (Chauvin)- Fluorescein sodium... 

FLUORESCEIN SODIUM OPHTHALMIC STRIPS MODIFIED 300S 6505011591493 PG 125,24 ... 

Fluorescein conjugates, 14 149 Fluorescein sodium, water treatment compound for aquaculture in U.S.,... 

Freezing point depression of 1% fluorescein sodium is 0.18°. Calculate the volume of iso-osmotic solution produced by 1 g of fluorescein by U.S.P. method. 

Fluorescein Sodium Dissolve 50 mg in 20 ml DW, and 2.5 ml dil. HC1 and filter. Perform the test with the filtrate. Complies with the limit test for Sulphates. 

Using this technique, substantivity (Table 7) was assessed using a negatively charged vinyl substrate that is often used to simulate skin (the anionic UCARMAG Binder 527 resin). Fluorescein, sodium salt, at 0.5% in water was added before and/or after a 0.9% saline wash (2 X 25 mL) step. If added later, then samples were rewashed to determine whether any substantive coating remained. 

Fluorescein Sodium Solution for Injeotion Ophthalmological diagnostic agent... 

Calcein [2, 7 -bis- A, A -di(carboxymethyl)aminomethyl fluorescein] sodium salt [1461-15-0] M 666.5. Dissolve in distilled H2O and acidify with dilute HCl to pH 3.5. Filter off the solid acid and wash well with H2O. Redissolve ca lOg in 300ml H2O containing 12g of NaOAc. Ppte again by adding HCl, filter and wash with H2O. Add the solid to 200ml of EtOH stir for Ih and filter. Repeat the EtOH wash and dry the bright yellow solid in a vacuum. This acid decomposes on heating at ca 180°. See below for the prepn of the Na salt. [AC 28 882 1956]. 

Dyes (Fluorescein sodium salt, Phloxine B) TT I (Walthall and Stark, 1999)... 

Walthall, W.K. and Stark, J.D. (1999) The acute and chronic toxicity of two xanthene dyes, fluorescein sodium salt andphloxine B, to Daphniapulex, Environmental Pollution 104 (2), 207-215. 

SAFETY PROFILE Poison by intravenous route. Moderately toxic by intraperitoneal route. Mutation data reported. When heated to decomposition it emits acrid smoke and irritating fumes. See also FLUORESCEIN SODIUM. 

SYNS AIZEN URANINE CALCOCID URANINE B4315 9-o-CARBOXYPHEN iX-6-HYDROXY-3-ISOX.ANTHONE, DISODIUM SALT CERTIQUAL FLUORESCEINE C.I. 766 C.I. ACID YELLOW 73 C.I. 45350 DISODIUM S.M.T D C YELLOW No. 8 DISODIUM-6-HY DROXY-3-OXO-9-XANTHENE-o-BENZOATE FLUORESCEIN SODIUM B.P FLUORESCEIN, soluble FLUOR-I-STRIP A.T. FUL-GLO FUNDUSCEIN FURANIUM HIDACID UIL XINE NCI-C54706 RESORCINOL PHTHALEIN... 

SODIUM SODIUM FLUORESCEIN SODIUM FLUORESCEINATE SODIUM SALT of HYDROXY-o-CARBOXY-PHENYL-FLUORONE SOLUBLE FLUORESCEIN SPIRO(ISOBENZOFURr N)-l(3H),9 -(9H)XANTHENE-3-ONE, 3, 6 -DIHYDROXY-DISODIUM SALT URANTN URANINE A EXTRA URANINE USP XII URANINE YELLOW 11824 YELLOW ... 

Benoxinate hydrochloride with fluorescein sodium Fluress 0.4% solution combined with 0.25% fluorescein sodium 1% chlorobutanol... 

Fluorescein sodium, 3, 6 -dihydroxyspiro[isobenzofuran-l(3H),9 -[9H]xanthen], C2oHioNa205, CAS number 518-47-8, is a yellow acid dye of the xanthene series. Its molecular weight is 376 Da, and its solubility in water at 15°C is 50% (i.e., it is freely soluble). It is generally formulated as its sodium salt (Figure 16-1). When exposed to light, fluorescein maximally absorbs light at approximately 493 nm and emits (fluoresces) at approximately 520 nm. Figure 16-2 illustrates the excitation and emission spectra of dilute fluorescein in phosphate buffer. 

Because fluorescein sodium can penetrate into many hydrogel contact lenses, the lenses become discolored, which raises bacterial growth issues and renders the lenses cosmetically objectionable. In addition, the boimd-ary between lens and tears becomes obscured, which precludes the use of fluorescein in soft contact lens fitting. Fluorexon, a molecule similar in fluorescent characteristics to that of fluorescein, is less readily absorbed by the soft lens material, which renders it useful in fitting and evaluating soft and hybrid design lenses. 

Morris PE Fluorescein sodium and indocyanine green uses and side effects. In Saine PJ, Tyler ME, eds. Ophthalmic photography. Boston Butterworth-Heinemann, 2002 137-165. 

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