Pyrosequencing

Sun, A., Ge, J., Siffert, W., and Frey, U. H. (2005) Quantification of allele-specific G-protein beta3 subunit mRNA transcripts in different human cells and tissues by Pyrosequencing. Eur. J. Hum. Genet. 13, 361-369. 

Van Booven, D. J. (2006) Pyrosequencing genotype storage techniques. Methods Mol Biol. 373, 177-186. 

Sanapareddy N, Hamp TJ, Gonzalez EC et al (2009) Molecular diversity of a North Carolina waste water treatment plant as revealed by pyrosequencing. Appl Environ Microbiol 75(6) 1688-1696... 

Kristiansson E, Pick J, Janzon A et al (2011) Pyrosequencing of antibiotic-contaminated river sediments reveals high levels of resistance and gene transfer elements. PLoS One 6(2) el7038... 

Haglund S, Lindqvist M, Aimer S et al. Pyrosequencing of TPMT alleles in a general Swedish population and in patients with inflammatory bowel disease. Clin Chem 2004 50 288-295. Erratum in Clin Chem 2004 50 788. 

It should be possible to develop either biochemical- or molecular-based tests for resistance in flatworms, as has been successfully used to detect insecticide resistance in insects. However, the necessary information is not available on the mechanism of resistance to be able to design tests of this type for use with trematodes or cestodes. When developed they will hopefully be based on real-time polymerase chain reaction (PCR) or pyrosequencing. 

Pyrosequencing utilizes the release of inorganic pyrophosphate from DNA polymerase during the addition of dNTPs onto a growing sequence. Each... 

Mechanism Dideoxy chain termination Pyrosequencing Sequencing by synthesis (SBS) Single molecule real-time SBS Semiconductor-based SBS... 

Not surprisingly, the cutaneous microbiome has been studied for its potential role in dermatologic conditions such as psoriasis, atopic dermatitis, and acne. Psoriasis, a chronic idiopathic inflammatory disease of the skin (170), has been associated with an overrepresentation of Firmicutes and underrepresentation of Actinobacteria when compared to both the unaffected skin of psoriatic patients and the skin of normal controls (171). Additionally, Pro-teobacteria were detected less frequently in psoriatic lesions compared to skin of healthy controls (171). Subsequent studies using pyrosequencing techniques confirmed that Actinobacteria were more abundant in controls compared to patients with psoriasis however, Proteobacteria were significantly higher in trunk skin samples from psoriatic patients compared to controls (172). 

Sequencing chemistry Reversible terminator Pyrosequencing Ligation Proton detection... 

Russom, A., Ahmadian, A., Andersson, H., Van Der Wijngaart, W., Lundeberg, J., Uhlen, M., Stemme, G., Nilsson, P., SNP analysis by allele-specific pyrosequenc-ing extension in a micromachined filter-chamber device. Micro Total Analysis Systems, Proceedings 5th pTAS Symposium, Monterey, CA, Oct. 21-25, 2001, 22-24. 

An alternative method of DNA sequencing, called Pyrosequencing (3) uses a series of enzymatic reactions to enable the continuous readout of short stretches of DNA sequence without the requirement for electrophoretic fragment separation. The method may be useful for low-throughput research and clinical diagnostic applications, but is unlikely to be applied to high-throughput applications. 

Fakhrai-Rad H, Pourmand N, Ronaghi M. Pyrosequencing an accurate detection platform for single nucleotide polymorphisms. Hum Mutat2002 19 479-485. 

Goldberg, S. M. D., Johnson, J., Busam, D., Feldblyum, T., Ferriera, S., Friedman, R., et al. (2006). A Sanger/pyrosequencing hybrid approach tor the generation of high-quality draft assemblies of marine microbial genomes. Proc. Natl. Acad. Sci. U. S. A 103, 11240-11245. 

Newer technologies that replace assays traditionally performed by electrophoresis are emerging, some of which are attractive alternatives for the clinical laboratory as they achieve analysis of nucleic acids with less hands-on time and with far greater throughput because of automation. These include pyrosequencing, mass spectrometry, and HPLC. 

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