Pyrosequencing polymerase chain reaction

It should be possible to develop either biochemical- or molecular-based tests for resistance in flatworms, as has been successfully used to detect insecticide resistance in insects. However, the necessary information is not available on the mechanism of resistance to be able to design tests of this type for use with trematodes or cestodes. When developed they will hopefully be based on real-time polymerase chain reaction (PCR) or pyrosequencing. 

Pyrosequencing produces specific sequence data in the form of peaks on a pyrogram. It does not require the presence of a restriction enzyme site, and polymerase chain reaction (PCR) product and internal primer sites can vary in size and position. In addition, it can be used to identify tri-allelic, indel, and short-... 

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