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Pyrosequencing technology

Wasson, J., Skolnick, G., Love-Gregory, L., and Permutt, M. A. (2002) Assessing allele frequencies of single nucleotide polymorphisms in DNA pools by pyrosequencing technology. Biotechniques 32, 1144-1146. [Pg.163]

Newer technologies that replace assays traditionally performed by electrophoresis are emerging, some of which are attractive alternatives for the clinical laboratory as they achieve analysis of nucleic acids with less hands-on time and with far greater throughput because of automation. These include pyrosequencing, mass spectrometry, and HPLC. [Pg.1427]

Pyrosequencing was designed by Hyman in 1988 and developed by Ronaghi el al., from the Stockholm Technological Institute, and it is one of the most widely NGS techniques used (29,30). It employs a library generated by emPCR as a DNA template for sequencing using nucleotides that are supplied in a predetermined order. When the introduced nucleotide is complementary to the template, the DNA polymerase is activated, the nucleotide is incorporated to the DNA chain and a pyrophosphate molecule is released. [Pg.54]

Pyrosequencing provides rapid real-time determination of 20-30 nucleotides of target DNA sequence. With this technology, not only the SNP alleles but also the... [Pg.678]

The technology company 454 Life sciences has developed a solid-phase parallel microarray system of microfluidic wells in which 10 oligonucleotide molecules per square centimeter are used to capture complementary DNA fragments. Pyrosequencing reactions are simultaneously monitored on each... [Pg.868]

Avantome, NexBio, ParAllele Bioscience, and Pyrosequencing AB. He received a Ph.D. from the Royal Institute of Technology in Sweden. [Pg.164]

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See also in sourсe #XX -- [ Pg.54 , Pg.56 , Pg.56 ]



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Pyrosequencing

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