Porcine pseudorabies virus

PRV porcine pseudorabies virus FVIII molecule, retaining the known func-... 

The virus reduction studies of the three process steps discussed here were performed with HFV-l, Bovine viral diarrhea virus (BVDV), Pseudorabies virus (PRV), Reovirus type 3 (Reo), Hepatitis A virus (HAV), and Porcine parvovirus (PPV). HIV-1 was included as a relevant enveloped virus, while BVDV and PRV were tested as specific model viruses for HCV and HBV, respectively (Table 1). Reo was chosen as a non-specific model non-enveloped virus, HAV was included as a relevant virus and PPV was used as a surrogate for human parvovirus B19. All viruses were propagated using standard cell culture conditions. " The appropriate cell lines were infected, at a low multiplicity of infection, and incubated until 4-1- cytopathic effects were observed. The infected cells were frozen and thawed three times to release virus, centrifuged at low speed to remove cell debris and the clarified supernatants were removed for use as virus spikes. 

Process step Pseudorabies virus Xenotropic murine retrovirus Human adenovirus Porcine parvovirus Poliovirus Mouse adenovirus ... 

The in vitro and in vivo effects of sulphated PS were also investigated against the pseudorabies virus (Suid HSV-1), the most notable of which is porcine HSV which has a well-documented history of epizootic infections, especially in Europe [105], In vitro experiments revealed that sulphated PS significantly reduced the number of lytic plaques, but in vivo only heparin protected mice against the pseudorabies virus. 

Ambagala AP, Hinkley S, Srikumaran S (2000) An early pseudorabies virus protein down-regulates porcine MHC class I expression by inhibition of transporter associated with antigen processing (TAP). J Immunol 164 93-99... 

Suggested Alternatives for Differential Diagnosis Classical swine fever, African swine fever, pseudorabies, rabies, Japanese encephalitis, edema disease, hemagglutinating encephalomyelitis, bacterial meningoencephalitis, porcine reproductive and respiratory syndrome (PRRS) virus, hypoglycemia, water deprivation/salt intoxication, and other toxins. 

Solutions of glutaraldehyde (2%) and formaldehyde (8%) have been shown to be effective against porcine parvovirus after 20 minutes, and pseudorabies and transmissible gastroenteritis viruses after 5 minutes exposure [169]. Aleutian disease virus of mink was inactivated by formalin only after 30 minutes exposure, but glutaraldehyde inactivated 4-log of infectious virus within 5 minutes at 23°C [170]. Formalin (0.2%) was found to be ineffective... 

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