Proteomics- definition

Page, M. J., Amess, B., Townsend, R. R., Parekh, R., Herath, A., Brusten, L., Zvelebil, M. J., Stein, R. C., Waterfield, M. D., Davies, S. C., and O Hare, M. J. (1999). Proteomic definition of normal human luminal and myoepithelial breast cells purified from reduction mammoplasties. Proc. Natl. Acad. Sci. USA 96, 12589-12594. 

Page MJ et al. Proteomic definition of normal human luminal and myoepithelial breast cells purified from reduction mammoplasties. Proc Natl Acad Sci USA 1999 96 12589-12594. 

Makanga, M., Bray, P. G., Horrocks, P., and Ward, S. A. (2005). Towards a proteomic definition of CoArtem action in Plasmodium falciparum malaria. Proteomics 5,1849-1858. 

Figure 7.6. (a) Definitions of success rate and relative dynamic range, (b) Model of a proteomics experiment. (See color insert.)... 

Le Bihan, T., Robinson, M. D., Stewart, 1. L, and Figeys, D., Definition and characterization of a trypsinosome from specific peptide characteristics by nano-HPLC-MS/MS and in silico analysis of complex protein mixtures. Journal of Proteome Research 3(6), 1138-1148, 2004. 

ECVAM is the leading international center for alternative test method validation. Hartung et al. (29) summarized the modular steps necessary to accomplish stage 3 (test validation). The seven modular steps are (I) test definition, (2) within-laboratory variability, (3) transferability, (4) between-laboratory variability, (5) predictive capacity, (6) applicability domain, and (7) performance standards (29). Steps 2-4 evaluate the test s reliability steps 5 and 6 evaluate the relevance of the test. Successful completion of all seven steps is necessary to proceed to stage 4 (independent assessment or peer review). This modular approach allows flexibility for the validation process where information on the test method can be gathered either prospectively or retrospectively. The approach is applicable not only to in vitro test methods but also to in silico approaches (e.g., computer-based approaches such as quantitative structure-activity relationships or QSAR) and pattern-based systems (e.g., genomics and proteomics). 

The contribution of mass spectrometry to the biosciences, especially in proteomics (the determination of the protein constituents of a cell or organism) of individual components and the quantitative definition of cellular processes, was reviewed by Gaskell.68... 

The success of the project to describe the human genome along with progress in the definition of polymorphisms in human xenobiotic-metabolizing enzymes and other proteins will certainly lead to the ability to define populations and individuals at increased risk from a particular chemical insult. This ability will be extended and put on a more mechanistic basis by advances in the new disciplines of proteomics and metabonomics. 

The problem many chemists have chosen to attack, then, is to find out how proteins are made naturally in cells and then develop methods for replicating those methods in the laboratory. This held of study has now been given the name proteomics. According to a definition suggested by Stanley Fields at the University of Washington, proteomics is the science that seeks to identify and quantify proteins and to determine localization, modifications, interactions, activities, and, ultimately, their functions. Knowledge of this kind is essential, of course, not only in understanding how natural proteins function,... 

Keough T, Lacey MP, Fieno AM, Grant RA, Sun Y, Bauer MD, Begley KB. Tandem mass spectrometry methods for definitive protein identification in proteomics research. Electrophoresis 2000 21 2252-2265. 

Proteomics and its possible applications for assessment of allergenicity of food products are described by Bannon et al. (2008), who draw attention to the fact that reproducibility of results obtained though proteomic techniques need to be improved by introducing more precise definitions of the parameters of technical determinations. Nonetheless, proteomics provides high-throughput methods for detection and characterization of proteins. 

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