Proteins high-performance liquid chromatography

Proteins High Performance Liquid Chromatography Reverse Phase Liquid Chromatography 44 ... [Pg.579]

Typically, quantitative protein determination is done on the one hand by colorimetric or nephelometric methods, on the other hand for more difficult analytical problems by more sophisticated techniques such as high performance liquid chromatography (HPLC), gel-electrophoresis and immunoassay. However, these methods are tedious, time-consuming and expensive. [Pg.100]

SynChropak GPC supports were introduced in 1978 as the first commercial columns for high-performance liquid chromatography of proteins. SynChropak GPC columns were based on research developed by Fred Regnier and coworkers in 1976 (1,2). The first columns were only available in 10-yu,m particles with a 100-A pore diameter, but as silica technology advanced, the range of available pore diameters increased and 5-yu,m particle diameters became available. SynChropak GPC and CATSEC occasionally were prepared on larger particles on a custom basis, but generally these products have been intended for analytical applications. [Pg.305]

APPLICATION OF SIZE EXCLUSION-HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY FOR BIOPHARMACEUTICAL PROTEIN AND PEPTIDE THERAPEUTICS... [Pg.531]

W. G. Button, K. D. Nugent, T. K. Slattery, B. R. Summers and L. R. Snyder, Separation of proteins by reversed-phase high-performance liquid chromatography , 7. Chromatogr. 443 363-379(1988). [Pg.213]

H. Yamamoto, T. Manabe and T. Okuyama, Apparatus for coupled high-performance liquid chromatography and capillary electrophoresis in the analysis of complex protein mixtures , 7. Chromatogr. 515 659-666 (1990). [Pg.214]

Aguilar, M. I., Hodder, A. N., and Hearn, M. T. W., High-performance liquid chromatography of amino acids, peptides, and proteins. LXV. Studies on the optimisation of the reversed-phase gradient elution of polypeptides. Evaluation of retention relationships with (3-endorphin-related polypeptides, /. Chromatogr., 327, 115, 1985. [Pg.54]

Torres, A. R., Edberg, S.C., and Peterson, E. A., Preparative high-performance liquid chromatography of proteins on an anion exchanger using unfractionated carboxymethyl displacers, /. Chromatogr., 389, 177, 1987. [Pg.127]

Lee, A. L., Liao, A. W., and Horvath, Cs., Tandem separation schemes for preparative high-performance liquid chromatography of proteins,. Chromatogr., 443, 31, 1988. [Pg.127]

Koyama, J., Nomura, J., Shiojima, Y., Ohtsu, Y., and Horii, I., Effect of column length and elution mechanism on the separation of proteins by reversed-phase high-performance liquid chromatography, /. Chromatogr., 625, 217, 1992. [Pg.191]

Lau, S. Y. M., Taneja, A. K., and Hodges, R. S., Effects of high-performance liquid chromatographic solvents and hydrophobic matrices on the secondary and quaternary structure of a model protein. Reversed-phase and size exclusion high-performance liquid chromatography, /. Chromatogr., 317, 129, 1984. [Pg.197]

Williams, R. C., Vasta-Russell, J. F., Glajch, J. L., and Golebiowski, K., Separation of proteins on a polymeric fluorocarbon high-performance liquid chromatography packing, /. Chromatogr., 371, 63, 1986. [Pg.198]

Berchtold, M. W., Heizmann, C. W., and Wilson, K. J., Ca2+-binding proteins a comparative study of their behavior during high-performance liquid chromatography using gradient elution in reverse-phase supports, Anal. Biochem., 129, 120, 1983. [Pg.198]

Sadler, A. J., Micanovic, R., Katzenstein, G. E., Lewis, R. V., and Middaugh, C. R., Protein conformation and reversed-phase high-performance liquid chromatography, /. Chromatogr, 317, 93, 1984. [Pg.198]

Coupek, J. and VinS, I., Hydroxyethyl methacrylate-based sorbents for high-performance liquid chromatography of proteins, /. Chromatogr. A, 658, 391, 1994. [Pg.198]

Regnier, E E. and Gooding, K. M., High-performance liquid chromatography of proteins, Anal. Biochem., 103, 1, 1980. [Pg.198]

Yang, Y.-B., Harrison, K., and Kindsvater, J., Characterization of a novel stationary phase derived from a hydrophilic polystyrene-based resin for protein cation-exchange high-performance liquid chromatography, /. Chromatogr. A, 723, 1, 1996. [Pg.280]

Josic, Dj., Hofmann, W., Wieland, B., Nuck, R., and Reutter, W., Anion-ex-change high-performance liquid chromatography of membrane proteins from liver and Morris hepatomas, /. Chromatogr., 359, 315, 1986. [Pg.280]

Imaoka, S. and Funae, Y., Ion-exchange high-performance liquid chromatography of membrane-bound protein cytochrome P-450,. Chromatogr., 375,83,1986. [Pg.280]

Cacia, J., Quan, C. P., Vasser, M., Sliwkowski, M. B., and Frenz, J., Protein sorting by high-performance liquid chromatography I. Biomimetic interaction chromatography of recombinant human deoxyribonuclease I on polyionic stationary phases, /. Chromatogr., 634, 229, 1993. [Pg.280]

Biinger, H., Kaufner, L., and Pison, U., Quantitative analysis of hydrophobic pulmonary surfactant proteins by high-performance liquid chromatography with light-scattering detection, /. Chromatogr. A, 870, 363, 2000. [Pg.381]

Capillary electrophoresis employing chiral selectors has been shown to be a useful analytical method to separate enantiomers. Conventionally, instrumental chiral separations have been achieved by gas chromatography and by high performance liquid chromatography.127 In recent years, there has been considerable activity in the separation and characterization of racemic pharmaceuticals by high performance capillary electrophoresis, with particular interest paid to using this technique in modem pharmaceutical analytical laboratories.128 130 The most frequently used chiral selectors in CE are cyclodextrins, crown ethers, chiral surfactants, bile acids, and protein-filled... [Pg.405]

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