Light-scattering detection

Fig. 11 Separation of phenacyl esters of the isomeric octadecenoic acids, petroselinic acid (6-18 1), oleic acid (9-18 1), and vaccenic acid (11-18 1) by HPLC on silver ion column (Nucleosil 5SA) loaded with silver ion eluted with dichloromethane-dichloroethane-acetonitrile 50 50 0. 25 (vol vol) detection light-scattering detector. (From W.W. Christie, Analusis 26 M38 (1998), reproduced with permission.)...

Light-scattering detection Light-scattering detector Known dn/dc as a function of elution Direct MMD measurement, No calibration. Architecture independence No CCD information. No TgS 5, 8... 

SEC with On-Line Triple Detection Light Scattering, Viscometry, and R( Susan V. Greene... 

However, despite this progress, it is highly recommended that both PCS and LD be used simultaneously. It should be kept in mind that both methods are not measuring particle sizes. Rather, they detect light-scattering effects that are used to calculate particle size. For example, uncertainties may result from nonspherical particle shapes and from the assumption of certain parameters that are used to calculate the particle size. Platelet structures commonly occur during lipid crystallization [57] and have also been observed for SLN [11,40,58], The presence of several populations and other colloidal structures adds further difficulties. 

Instrumentation. The experimental setup is similar to the one used in SPR. An additional photomultiplier mounted at fixed angle with respect to the base of the prism at the solution side of the electrode films is used to detect light scattered from the rough electrode surface and/or the polymer film deposited on the metal film (see Fig. 5.153). 

Light Scattering Detection Light scattering is one of the few absolute methods for the characterization of macromolecules and biopolymers. Online light scattering... 

The first of the modem diffraction based instruments for particle size analysis is probably that produced by CILAS (France). This is described by Comillautf and used a moveable mask to detect light scattered at different angles. The Leeds and Northrup Microtrac dates from 1976, this was described by Wiess and Frock . 

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