Post-transcriptional control

Dixon, D. A. et al. Post-transcriptional control of cyclooxygenase-2 gene expression. The role of the 3 -untranslated region. J. Biol. Chem. 275, 11750, 2000. 

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Lillo, C. (1991). Diurnal variations of corn leaf nitrate reductase an experimental distinction between transcriptional and post-transcriptional control. Plant Science 73, 149-54. 

Hadcock, J.R. and Malbon, C.C. (1991). Regulation of receptor expression by agonists transcriptional and post-transcriptional controls. TINS 6, 242 247. 

The most informative comparison is that of steady state mRNA levels to the relative transcription rate for each gene. In the case of both SSP genes, the difference in the relative transcription rate at 8 and 13 DPF is qualitatively similar to the relative steady state levels of mRNA. However the difference in the level of transcription between 8 DPF and 13 DPF is large compared to the difference in steady state mRNA levels between these points. This suggests that both transcriptional and post-transcriptional controls are operative. The latter possibly involves mRNA stability within the cell. In the case of the 8-LOX gene the transcriptional levels follow the same pattern as the steady state mRNA levels indicating almost exclusively transcriptional control of this gene. For both... 

Jacobs Anderson, J. S., and Parker, R. (2000) Computational identification of cis-acting elements affecting post-transcriptional control of gene expression in Saccharomyces cerevisiae. Nucleic Acids Res. 28, 1604-1617. 

In this section we consider other mechanisms of post-transcriptional control that contribute to regulating the expression of some genes. Most of these mechanisms operate in the cytoplasm, controlling the stability or localization of mRNA or its translation into protein. We begin by discussing two recently discovered and related mechanisms of gene control. 

Gibson, W. C., Swinkels, B. W. and Borst, P. (1988) Post-transcriptional control of the differential expression of phosphoglycerate kinase genes in Trypanosoma brucei. J. Mol. Biol. 201 315-325. 

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Zick A, Onn I, Bezalel R et al. Assigning functions to genes Identification of S-phase expressed genes in Leishmania major based on post-transcriptional control elements. Nucleic Acids Res 2005 33(13) 4235-42. 

Pantopoulos K, Weiss G, Hentze MW (1994) Nitric oxide and the post-transcriptional control of cellular iron traffic. Trends Cell Biol 4 82-86... 

A more dramatic prediction of the post-transcriptional control model is also substantiated in Figure 6. Following deinduction of a culture inhibition of RNA synthesis should rescue the repressed mRNA and reactivate enzyme formation. Experiments in accord with this prediction were done in HTC cells (Tomkins et al., 1969,1970 Levinson et al., unpublished), and in cultured fetal hamster cells (Bausserman and Nebert, 1970). The results are in remarkably good agreement. In the experiment using HTC cells (Fig. 6) the inducer was removed from the induced culture, and after specified intervals AMD was added to the deinduced culture. As illustrated, TAT formation was reactivated shortly after addition of the inhibitor. Similar results are obtained when the nucleoside analog mercaptopyridethylbenzimidazole (MPB) was used instead of actinomycin D (Levinson, unpublished Tomkins et al., 1970). 

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