Polymerase chain reaction screening

Berfstrome Jones AC, Doon A. Evaluation of a single-tube multiplex polymerase chain reaction screen for detection of common alpha thalassemia genotypes in a clinical laboratory Am J Clin Path 2002 118 18-24. 

The discovery and exploitation of enzymes in aldoxime-nitrile pathway nitrile hydratase, amidase, nitrilase, aldoxime dehydratase, etc., are shown along with the use of methodologies, such as organic chemistry, microbial screening by enrichment and acclimation culture techniques, enzyme purification, gene cloning, molecular screening by polymerase chain reaction (PCR). 

Oh, K.T., Anis, A.H., and Bae, S.C. (2004) Pharmacoeconomic analysis of thiopurine methyltransferase polymorphism screening by polymerase chain reaction for treatment with azathioprine in Korea. Rheumatology (Oxford). 43,156-163. 

The polymerase chain reaction (PCR), developed by Mullis, is a simple and most effective way of amplifying, i.e. producing multiple copies of, a DNA sequence. It finds applications in all sorts of areas not immediately associated with nucleic acid biochemistry, e.g. genetic screening, medical diagnostics, forensic science, and evolutionary biology. The general public is now well aware of the importance... 

Two types of screening procedures are used to identify ES cell clones carrying a targeted integration of the construct DNA polymerase chain reaction (PCR) and Southern blot analysis (Southern, 1975). Both methods rely upon the specific juxtaposition of vector components and target locus sequences after homologous recombination. 

Fig. 6. (Opposite page) A high-throughput production method for screening proteins from cDNA libraries. Authentic (A) and glutathione -transferase (GST)-fused (G) proteins in the reaction mixtures after a semi-automated polymerase chain reaction/ transcription and translation from 54 different cDNAs separated by sodium dodecyl sul-fide-polyaciylamide gel electrophoresis and stained with Coomassie Brilliant Blue. T and S, respectively, mark total translation product and the supernatant fraction after centrifugation at 30,000g for 15 min.

Sung, W.C., Lee, G.B., Tzeng, C.C., Chen, S.H., Plastic microchip electrophoresis for genetic screening The analysis of polymerase chain reactions products of fragile X (CGG)n alleles. Electrophoresis 2001, 22, 1188-1193. 

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