Polymerase chain reaction rearrangements

Komminoth, P., Long, A. A., Ray, R., and Wolfe, H. J. (1992) In situ polymerase chain reaction detection of viral DNA, single copy genes and gene rearrangements in cell suspensions and cytospins. Diag. Mol. Pathol. 1, 85-97. 

Gutzmer, R. Mommert, S. Kiehl, P. Wittmann, M. Kapp, A. Werfel, T. Detection of clonal T cell receptor gamma gene rearrangements in cutaneous T cell lymphoma by LightCycler-polymerase chain reaction. J. Invest. Dermatol. 2001,116(6), 926-932. 

Polymerase Chain Reaction Analysis of Antigen-Receptor Gene Rearrangements... 

Figure 39-7 Polymerase chain reaction (PCR) analysis for clonal rearrangements of the antigen-receptor gene loci. PCR analysis of theTCR y-chain locus is shown. The upper and middle panels show duplicate assays with monoclonal capillary electrophoretic peaks of identical size ( 166 bp) in both replicates.The bottom panel shows a polyclonal pattern.

Greiner TC, Raffeld M, Lutz C, Dick F, Jaffe ES. Analysis of T cell receptor-gamma gene rearrangements by denaturing gradient gel electrophoresis of GC-clamped polymerase chain reaction products. Correlation with tumor-specific sequences. Am J Pathol 1995 146 46-55. 

Detection of immunoglobulin K light chain rearrangements by polymerase chain reaction. An improved method for detecting clonal B-cell lymphoproliferative disorders. Am J Pathol, 155, 355-363. 

After identification ofthe spliceosome as the pladienohde target, sphcing inhibition under pladienohde B treatment could be confirmed by polymerase chain reaction (PCR)-based assays against sphced and unspliced isoforms of several genes. Furthermore, it was observed that pladienohde application led to rearrangement and enlargement of nuclear speckles. 

APL is characterized by the t( 15 17) yet, in a recent study of 100 patients entered into the UK Medical Research Council (MRC) ATRA trial for APL, conventional cytogenetics failed to identify this translocation in 13% patients in whom its molecular consequence, the PML-RARa rearrangement, was ultimately successfully found by reverse transcriptase-polymerase chain reaction (RT-PCR) (5) Inability to detect t(15 17) on karyotype assessment most com-... 

Miller, W H., Kakizuka, A., Frankel, S. R, Warrell, R P, DeBlasio, A., Levine, K., Evans, R., and Dmitrovsky, E. (1992) Reverse transcription polymerase chain reaction for the rearranged retinoic acid receptor a clarifies diagnosis and detects minimal residual disease in acute promyelocytic leukemia Proc Nat Acad Sci. USA 89,2694-2698. 

Subramanian D, Albrecht S, Gonzalez JM, et al. Primary pulmonary lymphoma. Diagnosis by immunoglobulin gene rearrangement study using a novel polymerase chain reaction technique. Am Rev Respir Dis 1993 148 222-226. 

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