Dynamic allele-specific hybridization

Prince JA, Feuk L, Howell WM, Jobs M, Emahazion T, Blennow K, Brookes AJ. Robust and accurate single nucleotide polymorphism genotyping by dynamic allele-specific hybridization (DASH) design criteria and assay validation. Genome Res 2001 11 152-162. 

Jobs M, Howell WM, Stromqvist L, Mayr T, Brookes AJ. DASH-2 flexible, low-cost, and high-throughput SNP genotyping by dynamic allele-specific hybridization on membrane arrays. Genome Res 2003 13 916-924. 

Dynamic allele-specific hybridization, a method to detect SNPs, is based on dynamic heating and coincident monitoring of DNA denaturation and avoids the use of additional enzymes or reaction steps. 

W. Matthias Howell, Magnus Jobs, Ulf Gyllensten and Anthony J. Brooks, Dynamic allele-specific hybridization, Nature Biotechnology, 17 (1999), 87-88. 

DASH, dynamic allele-specific hybridization AS-PCR, allele-specific polymerase chain reaction AS-PE, allele-specific primer extension APEX, arrayed primer extension FP-TDI, fluorescence polarization template directed dye terminator incorporation MALDI-TOF-MS, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry OLA, oligonucleotide ligation assay RCA, ... 

Flowell WM, etal. Dynamic allele-specific hybridization. A new method for scoring single-nucleotide polymorphisms. Nat Biotechnol 1999 17 87-88. 

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