Polymer proteins onto

R. G. Lee, S.W. Kim, Adsorption of protein onto hydrophobic polymer surfaces adsorption isotherms and kinetics, J. Biomed. Mater. Res. 8 (1974) 251-259. 

On the other hand, the values for g13 or g23 are positive, with a few exceptions. In other words, the adsorption processes of water and of protein onto polymer surfaces are for the most part thermodynamically unfavorable. The negative values of gs are the result of a remarkable decrease in free energy when adsorptive water molecules are driven back to the bulk water system, as seen... 

This separation is based on the size of the porous, hydrophobic gels. The pore size must be greater than the pore size of the molecules to be separated. Gel-permeation cleanup (GPC) is used for cleaning sample extracts from synthetic macromolecules, polymers, proteins, lipids, steroids, viruses, natural resins, and other high molecular weight compounds. Methylene chloride is used as the solvent for separation. A 5 mL aliquot of the extract is loaded onto the GPC column. Elution is carried out using a suitable solvent, and the eluate is concentrated for analysis. 

Adsorption of Blood Proteins onto Polymer Surfaces... 

A Ithough the adsorption of polymers onto solid surfaces has been thor-oughly studied (I), relatively few studies can be found in the literature on the adsorption of proteins onto polymer surfaces. In 1905, Landsteiner and Uhliz (2) discussed the interaction of serum proteins with synthetic surfaces. Blitz and Steiner (3) showed that albumin adsorption onto solid surfaces increased with increasing albumin concentration and that adsorption was nearly irreversible. Hitchcock reported (4) that adsorption of egg albumin onto collodion membranes followed a Langmuir isotherm with maximum adsorption occurring near the isoelectric point. Later, Kemp and Rideal (5) reported that protein adsorption onto solids conforms with Langmuir adsorption. 

The implication of such stimuli-responsive particles as a solid polymer support of biomolecules in the biomedical field is probably due to various factors (1) easiest to prepare via precipitation polymerization (hydrogel particles) or a combination of emulsion and precipitation polymerizations (core-shell particles), (2) the colloidal properties are related to the temperature and to the medium composition (i.e., pH, salinity, surfactant etc.), (3) the adsorption and the desorption of antibodies and proteins are principally related to the incubation temperature, (4) the covalent binding of proteins onto such hydrophilic and stimuli-responsive particles can be controlled easily by temperature, and, finally, (5) the hydrophilic character of the microgel particles is an undeniably suitable environment for immobilized biomolecules. 

To attain this goal, we used ATR techniques which have been described previously (6). A liquid ATR cell can be used to circulate protein solutions (or blood) through the cell while spectrally monitoring the adsorption of proteins onto the surface of the ATR crystal. In addition, the ATR crystal can be coated with a thin layer of polymer, permitting us to follow the adsorption of pro-... 

We can visualize that when our hair is rubbed with a plastic plate, strands of hair stand up from the scalp due to electrostatic repulsion among them. Similarly, when charged functional groups, such as diethylamino groups, are introduced onto the graft chains, the polymer chains extend from the pore surface due to their mutual repulsion. This extended polymer brush conformation provides the protein with three-dimensional binding sites. The multilayer binding of various proteins onto the ion-... 

Collagen and gelatin could be also crosslinked with WSC which has been widely used for covalent immobilization of proteins onto polymer matrixes (9). The water content of crosslinked gels is plotted as a function of WSC concentration in Figure 3. In this case, not only gelatin but also collagen showed a decrease in water content as the WSC concentration became higher. 

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