Polyethyleneimine coating

McNeff, C., Zhao, Q., Almlof, E., Flickinger, N., and Carr, P. W., The efficient removal of endotoxins from insulin using quaternized polyethyleneimine-coated porous zirconia, Anal. Biochem., 274, 181, 1999. 

T. O. Park, S. Cohen, and R. Langer, Controlled protein release from polyethyleneimine-coated poly(l-lactic acid)/pluronic blend matrices, Pharm. Res, 9, 37 (1992). 

Matrex PAE-300 (silica based, polyethyleneimine coated weak anion exchange, 10 fi particles) Polyvinyl sulfonic acid (2 kD) Ovalbumin, conalbumin 29... 

Shao et al. used polyethyleneimine-coated crystals to measure haemoglobin (bovine) directly in solution from 0.001 to 0.1 mgmL [142]. No interference was observed from BSA and the effect of different liquid depths over the crystal was also investigated. 

Polybutadiene coating, 127 Polyethyleneimine coating, 128 Polymer analysis, 131-132,147-163 Polymeric packings, 107-109,122-126,204-206 divinylbenzene-based packings, 122-124, 204-205... 

Manuel, W., Zheng, J., and Hornsby, R, Transfection by polyethyleneimine-coated microspheres, J. Drug Target, 9,15, 2001. 

L. casei in a repeated batch fermentation showed excellent biofilm stability for up to 72 days (Demirci and Pometto 1995). Using a different bioreactor configuration, L. casei cells on polyethyleneimine-coated foam glass beads yielded excellent results in a recycle batch fermentation. Both systems can produce an average of 4.3—4.6 g of lactic acid/l-h (Ho et al. 1997). 

Miscellaneous Applications. PEIs and their derivatives ate used as cementation auxihaties in cmde oil exploration (459), and for breaking cmde oil emulsions (460) in cmde oil extraction. Seed coatings of water-soluble copolymers containing polyethyleneimine have been developed (461). Polyethyleneimine derivatives have positive photoresist properties (462) amidated polyethyleneimines improve the flow properties of cement (463) and with few exceptions, A/-acyla2iddines act as chemical sterilisers for insects (464). 

Gellan/Polyethyleneimine (Hydroxyethyllated)/ (No Coating) T A/S Irregular Shape... 

Aziridine is an intermediate and monomer in the preparation of cationic polymers, such as polyaziridine (polyethyleneimine). These polymers are used to improve wet strength of paper, in fuel-oil and lubricant refining, as flocculating agents and in protective coatings, in textile finishing and for adhesives, polymer stabilizers, and surfactants (Lewis, 1993 Scherr et al., 1995). 

Levels of lactate in buttermilk and yoghurt (and blood) were estimated using disposable sensors formed from screen-printed graphite laminated between two polymer sheets [18]. Platinum (deposited by sputter-coating) was the transducing surface. Layers of Nation were added to reduce interference and were surmounted by lactate oxidase in a mixture of polyethyleneimine and poly (carbamoyl) sulphonate hydrogel. The samples were measured in stirred buffer. A good correlation between biosensor results and those obtained with an enzyme kit was claimed but the data had a considerable amount of scatter—if the enzyme kit is taken as the reference method then a more severe analysis of the biosensor results [33] would not have shown them in a... 

In applications where self-polishing is not possible, the combination of a microbe-repelling surface and a release system seems to be desirable. One example of a design for such a surface is shown in Fig. 8. The depicted coating is based on a hydrophilic polymer network that contains polyethyleneimine crosslinkers, which are capable of selectively taking up silver ions and acting as a template for silver nanoparticles [90], This reloadable co-network was surface-modified with PEG,... 

For the capsule measurements, glass substrates coated with polyethyleneimine were used on which the PSS-terminated capsules showed adhesion. The capsule force deformation characteristics of individual capsules were obtained by placing a capsule under the colloidal probe and pressing onto it. Special care was taken to press onto the pole of the capsule as described in more detail in [13]. Reference curves on hard substrates were obtained before and after the experiments to determine the inverse optical lever sensitivity (InvoLS) and to derive the absolute deformation of the capsules. In rare cases when the InvoLS was found to change during the measurement, the measured data was discarded. 

Carr and others have described the preparation and properties of polybutadiene (PBD) and polyethyleneimine (PEI), as well as aromatic polymer-coated and carbon-clad zirconia-based RP phases. The preparation of PBD-coated zirconia and the chromatographic evaluation of these phases have been described extensively by Carr, McNeff, and others [39 1]. From these studies, the authors conclude that at least for neutral analytes PBD zirconia-coated phases behave quite similar with respect to retention and efficiency compared to silica-based RP phases [42]. For polar and ionic analytes, however, substantial differences with respect to retention, selectivity, and efficiency have been reported [43]. 

PMMA itself does not possess ready-to-use fiinctional groups for covalent binding with biological molecules. The amine-terminated PMMA were often produced by immersing the freshly cleaned PMMA substrate into a 1.0 M ethylenediamine in dimethyl sulfoxide (DMSO) solution for 15 min at room temperature (115) or coated with a thin layer of polyethyleneimine (PEI) or polyallylamine hydrochloride (PAH). This was first treated in 1 N sodium hydroxide (NaOH) solution at 55 C for 30 min and then immersed in a PEI or PAH solution (0.2%, pH 7) at room temperature for 1 h (117). Tsai and Lin (2005) demonstrated that PEI-derivatized PMMA was used for the determination of alpha-fetoprotein by quartz crystal microbalance (QCM) (118). Furthermore, the amine-terminated PMMA could be generated by reacting with 10% hexamethylene diamine (HMD) (reaction shown in scheme 8.2) or 1,3-diaminopropane (DAP) in 100 mM borate buffer pH 11.5 for 2 h (119) or exposing to -lithioethylenediamine (120) (reaction shown in scheme 8.3). 

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