Chromatographic evaluation

Fig. 6-3. Principle of the chromatographic evaluation of the recognition properties of MIPs.

Ascah, T., Kallury, K., Szafranski, C. Corman, S., and Liu, F., Characterization and high performance liquid chromatographic evaluation of a new amide-functionalized reversed-phase column, /. Liq. Chromatogr. Relat. Technol, 19 (17,18), 3049, 1996. 

Elargitai, T., Kaida, Y., and Okamoto, Y., Preparation and chromatographic evaluation of 3,5-dimethylphenyl carbamoylated beta-cyclodextrin stationary phases for normal-phase high-performance liquid-chromatographic separation of enantiomers, J. Chromatogr., 628, 11, 1993. 

Meyer, C., Skogsberg, U., Welsch, N., and Albert, K., Nuclear magnetic resonance and high-performance liquid chromatographic evaluation of polymer-based stationary phases immobilized on silica. Anal. Bioanal. Chem., 382, 679, 2005. 

Janini, GM., Muschik, G.M., Schroer, J.A., and Zielinski, W.L., Gas-liquid-chromatographic evaluation and gas-chromatography mass spectrometric application of new high-temperature hquid-crystal stationary phases for polycyclic aromatic hydrocarbon separations. Ana/. Chem., 4S, 1879, 1976. 

Brocco, D., V. di Palo, and M. Possanzini. Improved chromatographic evaluation of alkanes in atmospheric dust samples. J. Chromatogr. 86 234-238, 1973. 

S Moret, LS Conte. High-performance liquid chromatographic evaluation of biogenic amines in foods. An analysis of different methods of sample preparation in relation to food characteristics. J Chro-matogr A 729 363-369, 1996. 

The extraction of two typical agricultural products from environmental matrices were chosen as examples for the operation of this system. Diuron, a phenylmethylurea, was freshly spiked onto Tama soil. This soil was characterized and shown to have 3.1% organic material and 14 % clay fraction. In addition, a phenyl metabolite of NUSTAR, a systemic fungicide, on wheat previously unextractable by SFE was extracted. The wheat sample was not classified for its chemical composition. Both samples were treated with radiolabeled compounds (E. I. du Pont de Nemours and Company, Du Pont Agricultural Products, Wilmington, DE) and extraction results are from liquid scintillation counting of the sample extract. Chromatographic evaluation of the Diuron from soil extracts has previously been published (2). 

V. Das Gupta, High-performance liquid chromatography chromatographic evaluation of aqueous vehicles for preparation of prednisolone and prednisone dosage forms, J. Pharm. Sci., 68 908 (1979). 

The gas-liquid chromatographic evaluation of n can obviously be performed on a single distribution C E but not on mixtures of distributions. Commercial surfactants always consist of mixed distribution. Only when the procedure is applicable, rt=Lxnrt is calculated from the observed distribution of the molecular fraction (x ) for various values of n. It should be checked that x =l. 

Henriksen, A.P. and Stahnke, L.H. 1997. Sensory and chromatographic evaluations of water soluble fractions from dried sausages. J. Agric. Food Chem. 45, 2679-2684. 

Gupta, P.N., Naqvi, A., Misra, L.N., Sen, T. and Nigam, M.C. (1984) Gas chromatographic evaluation of the essential oils of different strains of Amomum subulatum growing wild in Sikkim. Perfume Kosmet 65, 528-529. 

The methods to be outlined here can be used, with careful choice of conditions, to establish with certainty the structural characteristics of a presumed phosphatidylglycerol sample. In most studies, at least with mammalian cells, the amount of material available for assay will be small hence thin-layer chromatographic evaluation of the success of a procedure will be an invaluable aid. 

Carr and others have described the preparation and properties of polybutadiene (PBD) and polyethyleneimine (PEI), as well as aromatic polymer-coated and carbon-clad zirconia-based RP phases. The preparation of PBD-coated zirconia and the chromatographic evaluation of these phases have been described extensively by Carr, McNeff, and others [39 1]. From these studies, the authors conclude that at least for neutral analytes PBD zirconia-coated phases behave quite similar with respect to retention and efficiency compared to silica-based RP phases [42]. For polar and ionic analytes, however, substantial differences with respect to retention, selectivity, and efficiency have been reported [43]. 

The polymers were prepared using acetonitrile as diluent and AIBN (60 C) or ABDV (40°C) as initiators. Polymerisation was started at the indicated temperatures and allowed to proceed for 24 h at each temperature. In the chromatographic evaluation the mobile phase was acetonitrile/acetic acid 90/10 (v/v). Each enantiomer (10 nmol) was injected separately. Data from [9]. 

The chromatographic evaluation was done using acetonitrile as mobile phase. The polymers were prepared at 60°C, using isopropanol as diluent and at the pressures given in the table. 

The imprinting effects of MIPs prepared with optically active compounds as the print molecules are readily demonstrated by chromatographic evaluations. For example, when the L-enantiomer of an amino acid derivative is used as the print species, a column packed with the resulting polymer will retain the L-enantiomer longer than the o-enantiomer and vice versa when the o-enantiomer is used as the print molecule. Reference polymers prepared with the racemate or without print molecule will not be able to resolve the racemate. A steroselective memory is hence induced in the polymers by the print molecules and is in many cases very precise. 

Barksdale, J.M. Clark, C.R. Synthesis and liquid chromatographic evaluation of some chiral derivatizing agents for resolution of amine enantiomers. Anal. Chem. 1984, 56 (6), 958-961. 

Hu, Y.L. Feng, Y.Q. Da, S.L. Chromatographic evaluation of alkylphosphonic acid-modified ceria-zirconia in reversed-phase HPLC. J. Liq. Chromatogr. Relat. Technol. 

Weber, T.P. Jackson, P.T. Carr, P.W. Chromatographic evaluation of porous-carbon-clad zirconia microplates. Anal. Chem. 1995, 67, 3042-3050. 

Rosano TG. Liquid-chromatographic evaluation of age-related changes in the urinary excretion of free catecholamines in pediatric patients. Chn Chem 1984 30 301-3. 

Chromatographic evaluation of the labelled molecules before and after purification was carried out using ITLC-SG or Whatman 3MM chromatography paper in different mobile phases, namely, 2-butanone (MEK), 0.9% NaCl, EtOH 0.01N HCl (90 10 vol./vol.) and butanokacetic acid H2O (4 1 5) [16.6]. The labelled molecules were also characterized by electrophoresis using Whatman No. 1 paper in Tris buffer (pH7.0) at 200 V, 80 mA for a period of 30 min. 

De Nardi, C. and Bonelli, F., Moving from fast to ballistic gradient in liquid chromatog-raphy/tandem mass spectrometry pharmaceutical bioanalysis Matrix effect and chromatographic evaluations, Rapid Commun. Mass Spectrom., 20(18), 2709, 2006. 

Moore, K.H.P. Brouwer, K.L.R. High-performance liquid chromatographic evaluation of the effect of heat treatment on trimethoprim and sulfamethoxazole stability in serum. Ther.Drug Monit., 1995, 17, 356-360... 

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