Poly P-hydroxybutyrate production

Bitar, A. and Underhill, S. (1990). Effect of ammonium supplementation on poly-P-hydroxybutyrate production by A. eutrophus in batch culture. Biotechnology Letter 12, 563-568. 

Choi, J and Lee, S. Y. (1999). Process analysis and economic evaluation for Poly-P-hydroxybutyrate production by fermentation. Bioprocess En neering 17,335-342. 

Jiang, Y, Song, X., Gong, L., Li, R, Dai, C., and Shao, W. (2008). High poly(P-hydroxybutyrate) production by Pseudomonas fluorescens A2a5 from inexpensive substrates. Enzyme andMicro-bial Technology 4,2, 167-172. 

Sayyed, R. Z. and Gangurde, N. S. (2010). Poly-P-hydroxybutyrate production by Pseudomonas sp. RZS 1 under aerobic and semi-aerobic condition. Indian Journal of Experimental Biology 48, 942-947. 

Poly-p-hydroxybutyrate (PHB) is a biodegradable thermoplastic that is produced by several microorganism. The PHB synthesis has been characterized in Alcaligens eutrophus and the operon involved in PHB production has been cloned. Recombinant E. coli strains that can produce high levels of PHB have been constmcted (39). 

Another example where metabolic pathway engineering has made a dramatic impact is in the biodegradable polymer field. One of the most widely studied polymers in this family is poly-P-hydroxybutyrate (PHB) (64). A related member of the poly-P-hydroxyalkanoate (PHA) family commercialized by Imperial Chemical Industries (ICI), which later became Zeneca Bio Products,... 

The enzymatic hydrolysis of poly-p-hydroxybutyrate, PHB, by several different bacteria, which are known to secrete active esterases, has been studied in some detail by several research groups [7, 8]. As with the polysaccharides, the final products of these degradation reactions are the monomers, dimers and trimers, which are removed by hydrolysis only from hydroxyl-end of the polymer chain, as follows ... 

Suzuki, T, Yamane, T., Shimizu, S. (1986). Mass production of poly-p-hydroxybutyric acid by fully automatic fed-batch culture of methylotroph. Applied Microbiology and Biotechnology, 23(5), 322-329. 

Jarute G, Kainz A, SchroU G, Baena JR, Lendl B (2004) On-line determination of the intracellular poly(P-hydroxybutyric acid) content in transformed Escherichia coli and glucose during PHB production using stopped-flow attenuated total reflection FT-IR spectrometry. Anal Chem 76 6353-6358... 

Chiellini E., Solaro R., Casini E., Casella S., Leporini C., Picci G., 1989, Biosynthetic degradable polymers. Study of the activity of Rhizobium "hedysarf strains in the production of poly(P-hydroxybutyrate). J. Bioact. Compat. Polym. 4 296-303. 

H., and Harrison, S.T.L. (2007) Environmental analysis of plastic production processes comparing petroleum-based polypropylene and polyethylene with biologically-based poly-P-hydroxybutyric add using life cyde analysis./. Biotechnol, 130 (1), 57-66. 

Lillo, J.A.G. and Rodriguez-Valera, F. (1990) Effects of culture conditions on poly (p-hydroxybutyric acid) production by Haloferax mediterranei. Applied Environmental Microbiology, 56, 2517-2521. Quillaguaman, J., Guzman, H., Van-Thuoc, D. and Kaul, R.H. (2010) Synthesis and production of polyhydroxyalkanoates by halophQes current potential and future prospects. Applied Microbiology and Biotechnology, 85,1687-1696. 

Young, F.K., James, R., Sheldon, K. and May, W. (1994) Microbial production of poly-P-hydroxybutyric acid from d-Xylose and lactose by Pseudomonas cepacia. Applied and Environmental Microbiology, 60(11), 4195-4198. 

Ishizaki, A. and Tanaka, K. (1991) Production of poly-p-hydroxybutyric acid from carbon dioxide by Alcaligenes eutrophus ATCC 17697T. Journal of Fermentation and Bioengineering, 4, 254-257. 

Yilmaz M, Soran H, Beyatli Y (2005) Determination of poly-P-hydroxybutyrate (PHB) production by some Bacillus spp. World J Microbiol Biotechnol 21 565-566 Zhao HY, Li HM, Qin LF, Wang HH, Chen G-Q (2007) Disruption of the polyhydroxyalkanoate synthase gene in Aeromonas hydrophila reduces its survival ability under stress conditions. FEMS Microbiol Lett 276 34-41... 

Braunegg G, Bogensberger B (1985) Zur Kinetik des Wachstums und der Speicherung von Poly-D(-)-3-hydroxybuttersaure bei Alcaligenes latus. Acta Biotechnol 5 339-345 Byrom D (1992) Production of poly-P-hydroxybutyrate poly-P-hydroxyvalerate copolymers. FEMS Microbiol Rev 103 247-250... 

Rusendi D, Sheppard JD (1995) Hydrolysis of potato processing waste for the production of poly-P-hydroxybutyrate. Bioresour Technol 54 191-196 Ruth K, Grubelnik A, Hartmann R, Egli T, Zinn M, Ren Q (2007) Efficient production of (R)-3-hydroxycarboxyUc acids by biotechnological conversion of polyhydroxyalkanoates and their purification. Biomacromolecules 8 279-286... 

Byrom D (1992) Production of poly-P-hydroxybutyrate and poly-p-hydroxyvalerate copolymers. FEMS Microbiol Rev 103 247-250... 

Page WJ, Knosp O (1989) Hyperproduction of poly-P-hydroxybutyrate during exponential growth of Azotobacter vinelandii UWD. Appl Environ Microbiol 55 1334-1339 Park WH, Lenz RW, Goodwin S (1998a) Epoxidation of bacterial polyesters with unsaturated side chains. I. Production and epoxidation of polyesters from 10-undecenoic add. Macromolecules 31 1480-1486... 

Park SJ, Park JP, Lee SG (2002) Production of poly(3-hydroxybutyrate) from whey by fed-batch culture of recombinant Escherichia coli in a pQot-scale fermenter. Biotechnol Lett 24 185-189 Pearce R, Marchessault RH (1994) Multiple melting in blends of isotactic and atactic poly(P-hydroxybutyrate). Polymer 35 3990-3997... 

Saruul P, Srienc F, Somers DA, Samac DA (2002) Production of a biodegradable plastic polymer, poly-beta-hydroxybutyrate, in transgenic alfalfa. Crop Sci 42 919-927 Schubert P, Steinbuchel A, Schlegel HG (1988) Cloning of the Alcaligenes eutrophus genes for the synthesis of poly-P-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli. J Bacteriol 170 5837-5847... 

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