Podophyllin

Common Name Podophyllinic acid 2-ethylhydrazide Structural Formula oh... 

The product is hydrogenated in 4,000 cc of ethanol at room temperature and under normal atmospheric pressure with a catalyst prepared In the usual manner from 400 g of Raney nickel alloy. The calculated amount of hydrogen is taken up in approximately 75 hours. After filtration and evaporation to a small volume, the residue Is distributed between 1,000 cc of chloroform and water each. The chloroform solution is then dried over sodium sulfate and evaporated to a small volume. Precipitation of the hydrogenation product with petroleum ether yields an amorphous white powder which Is filtered by suction, washed with petroleum ether and dried at 50°C In a high vacuum. 1. athyl-2-podophyllinic acid hydrazide is obtained in a practically quantitative yield. 

In addition to effects on biochemical reactions, the inhibitors may influence the permeability of the various cellular membranes and through physical and chemical effects may alter the structure of other subcellular structures such as proteins, nucleic acid, and spindle fibers. Unfortunately, few definite examples can be listed. The action of colchicine and podophyllin in interfering with cell division is well known. The effect of various lactones (coumarin, parasorbic acid, and protoanemonin) on mitotic activity was discussed above. Disturbances to cytoplasmic and vacuolar structure, and the morphology of mitochondria imposed by protoanemonin, were also mentioned. Interference with protein configuration and loss of biological activity was attributed to incorporation of azetidine-2-carboxylic acid into mung bean protein in place of proline. 

Available as a 0.5% gel or solution containing purified extract of the most active compound of podophyllin Arrests the formation of the mitotic spindle, prevents cell division, and may also induce damage in blood vessels within the warts... 

A 10% to 25% solution of podophyllin resin has been the standard in-office treatment for genital warts. 

Topical podophyllin is applied once weekly and the area should be allowed to dry. Immediately following treatment, the dried drug should be removed using alcohol or soap and water. Contraindicated in pregnant patients. 

Pregnancy Agents contraindicated in pregnancy include podofilox, fluorouracil, and podophyllin. Imiquimod is not approved for use in pregnancy, although it has been considered after signed consent has been obtained. Bichloroacetic and trichloroacetic acid have been used without problems. Ablative therapy is also a viable option. 

Podophyllin 10-25% in compound tincture of benzoin applied to lesions repeat weekly if necessary,1 or... 

Tome experts recommended washing podophyllin off after 1-4 hours to minimize local irritation. "Safety during pregnancy is not established. 

Hartwell, J. L. and M. J. Shear. 1947. Chemotherapy of cancer. Classes of compounds under investigation and active components of podophyllin. Cancer Res. 7 716-717. 

Podophyllum Basin. U.S.P. (Podophyllin) is prepared from the dried rhizome and roots of Podophyllum peltatum (may apple, mandrake), a plant used by native peoples. An amorphous greenish-yellow powder, with a slight peculiar odor and faintly bitter taste, its active constituents are a resin and a crystalline principle, podophyllotoxin. The dose of the resin is 0.01 to 0.065 g, 1/6 to 1 grain. It is very irritating to mucous membranes, especially of the eyes. 

Podophyllotoxin 1-0-glucoside (= Podophyllinic acid lactone 1-0-glucoside) (lignan)... 

Cryotherapy with liquid nitroget or podophyllin resin 10-25% in impound tincture of benzoin applied at weekly interoals ... 

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