Photobacterium leiognathi

Kulinski, T., Visser, A. J. W. G., O Kane, D. J., and Lee, J. (1987). Spectroscopic investigations of the single tryptophan residue and of riboflavin and 7-oxolumazine bound to lumazine apoprotein from Photobacterium leiognathi. Biochemistry 26 540-549. 

Petushkov, V. N., Gibson, G. B., and Lee, J. (1995). Properties of recombinant fluorescent proteins from Photobacterium leiognathi and their interaction with luciferase intermediates. Biochemistry 34 3300-3309. 

The (Cu,Zn)-SOD is found in the cytosol of eukaryotic cells and in the intermembrane space of mitochondria, as observed with chicken liver and also in chloroplasts, as shown with spinach and mustard leaves. A (Cu,Zn)-SOD has also been isolated from a prokaryote Photobacterium leiognathi. Could this symbiotic bacterium have borrowed the gene(s) from its host, the Pony fish, although two different subunits (Af 15,000 and 17,000) were reported for the bacterial enzyme A (Cu,Zn)-SOD was furthermore detected in Paracoccus denitrificans... 

Photobacterium leiognathi Gene mutation No data + Levi et al. 1986 H... 

F. Lavelle, M.E. McAdam, E.M. Fielden, E. Martin, P.B. Roberts, K. Puget, A.M. Michelson (1977). A pulse-radiolysis study of the catalytic mechanism of the iron-containing superoxide dismutase from Photobacterium leiognathi. J. Biochem., 161, 3-11. 

EFFECTS OF ORGANIC SOLVENTS ON BIOLUMINESCENCE EMISSION SPECTRA OF BACTERUL LUCIFERASE FROM PHOTOBACTERIUM LEIOGNATHI... 

Tyulkova N. Purification of bacterial luciferase from Photobacterium leiognathi with use FPLS-system. In Jezowska-Trzebiatowska B. ed. Biological luminescence. Singapore World Scientific, 1989 369 -74. 

In this work the interaction of bacterial luciferase from Photobacterium leiognathi with the flavin mononucleotide activated on phosphate group by N-methylimidazole, without addition of the exogenous aldehyde was study. 

In contrast to the bacterial-squid story, little is known about the horizontal transfer of luminous symbionts in bacteria-fish symbioses. Recently, however, we successfully raised Leiognathus nuchalis, pony fish juveniles, and infected them with symbiotic Photobacterium leiognathi. Juvenile L nuchalis raised separately from adults are apo-symbiotic and therefore not luminous. When the juveniles were kept with adults, a significant number became infected and luminescent. These results strongly support, the horizontal transfer of expelled P. leiognathi. 

Dunlap PV, McFall-Ngai Ml. Initiation and control of the bioluminescent symbiosis between Photobacterium leiognathi and leiognathid fish. In Lee JJ, Frederick JF. eds. Endocytobiology. III. New York New York Academy of Sciences, 1987 269-83. 

RFLP ANALYSIS OF THE LUX A GENES OF PHOTOBACTERIUM LEIOGNATHI ISOLATES DERIVED FROM THE SYMBIOTIC LIGHT ORGAN OF LEIOGNATHID FISH, LEIOGNATHUS RIVULATUS... 

Dunlap PV, Steinman, HM. Strain variation in bacteriocuprein superoxide dismutase from symbiotic Photobacterium leiognathi. J Bacteriol 1986 165 393-8. 

Dunlap PV, Jiemjit A, Ast JC, Pearce MM, Marques RR, Lavilla-Pitogo CR. Genomic polymorphism in symbiotic populations of Photobacterium leiognathi. Environ Microbiol 2004 6 145-58. 

Entrapment of luciferase from Photobacterium leiognathi in starch gels increases it s K , for dodecanal and tetradecanal to 1/3, but the change is insignificant for decanal. Also, K of aldehydes with different chain length is smaller for immobilized luciferase than for the soluble enzyme. ... 

Purification of luciferase. Luciferase was isolated from the recombinant strain of Echerichia coli SL60 with lux A and B genes of Photobacterium leiognathi from the collection of the Institute of Biophysics (RAS, SB) and purified by ion-exchange chromatography.5 The purity of the luciferase according to Laemmli electrophoresis was 90-95%. 

Bioluminescent enzymes bacterial luciferase from Photobacterium leiognathi and apo-obelin from the marine hydroid polyp Obelia longissima were purchased from the Photobiology Laboratory (Institute of Biophysics, SB RAS, Krasnoyarsk, Russia). Anthracene derivatives (2-chloranthracene (CA), 9-bromanthracene (BA)... 

Kudryasheva NS, Nemtseva EV, Visser AJWG, van Hoek A. Interaction of aromatic compounds with Photobacterium leiognathi luciferase fluorescence anisotropy study. Luminescence 2003 18 156-61. 

The dismutation of O2 by iron superoxide dismutase was found to be similar to that for the copper/zinc bovine superoxide dismutase. The results obtained by Lavelle et al. (1977) showed that catalysis of dismutation of O2 by the iron superoxide dismutase from Photobacterium leiognathi is first order with respect to substrate concentration for all ratios of substrate to enzyme concentrations reported. Although the enzyme is stable between pH 6.0 and 10.0, the value of the rate constant decreases as the pH increases. The second-order rate constant for the reaction be-... 

An X-ray structure for the manganese superoxide dismutase from Thermus aquaticus (Stallings, et al., 1985) and a complete amino acid sequence for the iron superoxide dismutase from Photobacterium leiognathi (Barra, et al., in press) confirm that the manganese and iron superoxide dismutases are homologous proteins. 

Scheme 17.14 Synthesis of neo-sialocon- transfer to lactoside acceptor 36, respectively jugates by using a one-pot, two-step reaction [33, 34]. For reactions with sialic acid subcascade using CSS from Neisseria meningitidis strates carrying bulky N-acyl groups, the CSS and a2,6-SiaT from Photobacterium leiognathi variants engineered for that purpose were JT-SHIZ-145 for sialic acid activation and employed.

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