Photobacterium leiognathi luciferase

Petushkov, V. N., Gibson, G. B., and Lee, J. (1995). Properties of recombinant fluorescent proteins from Photobacterium leiognathi and their interaction with luciferase intermediates. Biochemistry 34 3300-3309. 

EFFECTS OF ORGANIC SOLVENTS ON BIOLUMINESCENCE EMISSION SPECTRA OF BACTERUL LUCIFERASE FROM PHOTOBACTERIUM LEIOGNATHI... 

Tyulkova N. Purification of bacterial luciferase from Photobacterium leiognathi with use FPLS-system. In Jezowska-Trzebiatowska B. ed. Biological luminescence. Singapore World Scientific, 1989 369 -74. 

In this work the interaction of bacterial luciferase from Photobacterium leiognathi with the flavin mononucleotide activated on phosphate group by N-methylimidazole, without addition of the exogenous aldehyde was study. 

Entrapment of luciferase from Photobacterium leiognathi in starch gels increases it s K , for dodecanal and tetradecanal to 1/3, but the change is insignificant for decanal. Also, K of aldehydes with different chain length is smaller for immobilized luciferase than for the soluble enzyme. ... 

Purification of luciferase. Luciferase was isolated from the recombinant strain of Echerichia coli SL60 with lux A and B genes of Photobacterium leiognathi from the collection of the Institute of Biophysics (RAS, SB) and purified by ion-exchange chromatography.5 The purity of the luciferase according to Laemmli electrophoresis was 90-95%. 

Bioluminescent enzymes bacterial luciferase from Photobacterium leiognathi and apo-obelin from the marine hydroid polyp Obelia longissima were purchased from the Photobiology Laboratory (Institute of Biophysics, SB RAS, Krasnoyarsk, Russia). Anthracene derivatives (2-chloranthracene (CA), 9-bromanthracene (BA)... 

Kudryasheva NS, Nemtseva EV, Visser AJWG, van Hoek A. Interaction of aromatic compounds with Photobacterium leiognathi luciferase fluorescence anisotropy study. Luminescence 2003 18 156-61. 

Abbreviations for cell strains are as follows Vh, Vibrio harveyi Vf, Vibrio fischeri Plu, Photorhabdus luminescens. Pie, Photobacterium leiognathi Pp, Photobacterium phosphoreum. The bacterium strain is followed by the designation of the luciferase subunit. Active luciferase heterodimers are indicated by ++ for the native enzymes and + for the hybrid enzymes. Inactive hybrids are indicated by the negative sign. 

Aknashanu, S., Gendler, I., Hadar, R., and Kuhn, J., Interspecific luciferase beta subunit hybrids between Vibrio harveyi. Vibrio fischeri and Photobacterium leiognathi. Protein Eng., 9, 803,1996. 

The lyophilized luminous bacteria and lyophilized mixture of luciferase (Lu) from Photobacterium phosphoreum and NADHrFMN-oxidoreductase (R) from P. leiognathi were produced by the Biotechnology sector of the Institute of Biophysics (Krasnoyarsk). One vial of enzymes contained 0.11 mg of Lu and 0.069 units of activity/mL of R. One unit of R activity was defined as 1 mol of NADH degraded per min. All the assays were performed in the 0.1 mol/L phosphate buffer solution at pH 6.8 at room temperature. 

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