Echerichia coli

Human insulin was the first animal protein to be made in bacteria in a sequence identical to the human pancreatic peptide. Expression of separate insulin A and B chains were achieved in Echerichia coli K-12 using genes for the insulin A and B chains synthesized and cloned in frame with the... 

Pareilleux, A. and Sicard, N. (1970) Lethal effects of electric current on Echerichia coli. Appl. Environ. Microbiol. 19, 421 424. 

Ariga et al. [48] have investigated the behavior of the monolith reactor in which Echerichia coli with P-galactosidase or Saccharomyces cerevisiae was immobilized within a thin film of K-carragcenan gel deposited on the channel wall. The effects of mass transfer resistance and axial dispersion on the conversion were studied. Those authors found that the monolith reactor behaved like the plug-flow reactor. The residence-time distribution in this reactor was comparable to four ideally mixed tanks in series. The influence of gas evolution on liquid film resistance in the monolith reactor was also investigated. It was shown that at low superficial gas velocities, the gas bubble may adhere to the wall, which decreases the effective surface area available for the reaction. The authors concluded that the reactor was very effective in the reaction systems accompanied by gas evolution, such as fermentations. 

Purification of luciferase. Luciferase was isolated from the recombinant strain of Echerichia coli SL60 with lux A and B genes of Photobacterium leiognathi from the collection of the Institute of Biophysics (RAS, SB) and purified by ion-exchange chromatography.5 The purity of the luciferase according to Laemmli electrophoresis was 90-95%. 

Xu B, Jahic M, Bomsten G, Enfors S O (1999). Glucose overflow metabolism and mixed acid fermentation in aerobic large-scale fed-batch processes with Echerichia coli. Appl. Microbiol. Biotechnol. 51 564-571. 

Degussa/Rexim, partly in collaboration with Chinese manufacturers, produce (L)-threonine, (L)-valine and (L)-isoleucine by fermentation. [62] With genetically modified strains from Echerichia coli and Serratia marcescens, they are able to achieve end concentrations of 100 grams per litre. 

Von Wachenfeldt C, Richardson TH, Cosme J, Johnson EF (1997) Microsomal P450 2C3 is expressed as a soluble dimer in Echerichia coli following modifications of its N-terminus. Arch Biochem Biophys 339 107-114... 

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