Pheochromocytoma cells differentiation

PC 12 rat pheochromocytoma cells differentiated with NGF compared with C6 rat glioma cells differentiated with dBcAMP(Li and Casida, 1998)... 

PC 12 ral pheochromocytoma cells differentiated with NGF or undifferentiated fCrumpton et al, 2000)... 

Harada, H., Sugiyama, T., Suketa, Y. (1996). Characterization of inhibition by chronic treatment with lithium ion on nerve growth factor-induced neuronal differentiation on frat PC12 pheochromocytoma cells. Journal of Toxicology and Environmental Health, 49, 197-206. 

Grifinan, M., Soreq, H. (1997). Differentiation intensifies the susceptibility of pheochromocytoma cells to antisense oligodeoxynucleotide-dependent suppression of acetylcholinesterase activity. Antisense Nucl. Acid Drug Dev. 7 351-9. 

Fig. 1. Pertussis toxin-mediated ADP ribosylation of membrane G proteins. Isolated cell membranes (50 ng of protein) from N1E 115 cells (mouse neuroblastoma cell line), N2A cells (mouse neuroblastoma cell line), S49-1 eye cells (S49(-) mutated mouse lymphoma cell line deficient in Ga ), 549 wt cells (wild-type mouse lymphoma cell line), RBL (RBL 2H3 rat basophilic leukemia cell line), GH3 cells (GH3 rat hypophyseal tumor cell line), PC-12 (rat pheochromocytoma cell line), HIT-T15 cells (hamster insulinoma cell line), Y-1 cells (mouse adrenal cortex tumor cell line), 108 cc 15 cells (mouse/rat neuroblastoma x glioma hybrid cell line), HL-60 cells (DMSO-differentiated human leukemia cell line), HL-60 (+PT) cells (HL-60 cells pretreated with 25 ng/ml of pertussis toxin for 24 h prior to preparation of membranes), RINm5F cells (rat insulinoma cell line), and C6-2 cells (rat glioma cell line) were subjected to P-ADP-ribosylation as described in section 4.3.3. Samples were precipitated as outlined in section 4.3.5 and subjected to SDS-PAGE with separating gels containing 8% acrylamide (w/v). An autoradiogram of the dried gel is shown. Molecular masses of marker proteins are indicated (kDa). Modified Ga proteins migrate at approximately 40 kDa. Radioactivity running in front of the 30 kDa marker protein comigrates with the dye front...

Labelling of the cell surface of PC 12 pheochromocytoma cells for the presence of poly-A-acetyllactosamine chains identifies a restricted number of glycoproteins as carriers of these glycans (Fig. 7). Induced neuronal differentiation of the cells in culture... 

NGF inhibits proliferation and induces differentiation in PC 12 cells (isolated from a pheochromocytoma) and SH-SY5Y cells (isolated from a neuroblastoma [109]. The signal brought by NGF leads to iNOS activation, then to NO-mediated p21 activation [148] following two pathways NGF - iNOS - p53 - p21WAF1 and/or NGF - iNOS - p21WAF1 [109]. Neuroblastoma cell differentiation is induced by TNFa via iNOS expression [149]. 

Greene, L.A. (1978) Nerve growth factor prevents the death and stimulates the neuronal differentiation of clonal PC 12 pheochromocytoma cells in serum-free medium. J. Cell Biol. 78 747-755. 

Bisogno, T., Katayama, K., Melck, D., Ueda, N., De Petrocellis, L., Yamamoto, S., Di Marzo, V. (1998) Biosynthesis and degradation of bioactive fatty acid amides in human breast cancer and rat pheochromocytoma cells — implication for cell proliferation and differentiation. Eur. J. Biochem. 254, 634-642. 

Ong W, Jiang B, Tang N, Ling S, Yeo J et al. (2006) Differential effects of polyunsaturated fatty acids on membrane capacitance and exocytosis in rat pheochromocytoma-12 cells. Neurochem Res 31 41-8... 

Other cell lines are available for in vitro studies, including the Y1 mouse adrenocortical cell line, with different advantages and disadvantages (for review, see [49]). PC-12 cells are from a rat pheochromocytoma, and they produce norepinephrine and dopamine [60], They have been used to study neuroendocrine signaling of the adrenal medulla and differentiate and stop proliferating following exposure to neural growth factor. Adrenal slices have also been used for short-term experiments, especially those that required the presence of both medulla and cortex, but this model has rarely if ever been used to study toxicity of endocrine disruptors. 

Before the word proteomics appeared, the use of 2DE for studying growth factors mechanism of action was initiated with pheochromocytoma PC 12 cells (Hondermarck et al, 1994). In these cells, early protein synthesis induced by the differentiating activity of NGF and FGF-2 has been compared to the mitogenic activity of epidermal growth factor (EGF) using... 

PPy-PLGA electrospun meshes were used to support the growth and differentiation of rat pheochromocytoma 12 (PC12) cells and hippocampal neurons compared to noncoated PLGA meshes. It was suggested that PPy-PLGA may be suitable as conductive nanofibers for neuronal tissue scaffolds. 

Mammalian cells need some metals as nutrients to maintain functions such as proliferation, metabolism, and differentiation. Optimal metal concentrations vary with the types of cell. For example, estimates of the free zinc ion intra cellular concentration are 5-10 pM for pheochromocytoma (PC-12) cells, 170 pM for primary human monocytes and 350 pM for lymphocytes [6]. In addition, the regulating mechanism of metals is also diverse in cells. Mammalian cells have two ways for metal uptake and ejection one is endocytosis, another one is through metal transporters that can transport specified metal(s) selectively. Several kinds of transporters are known. Zinc transporters have two families ZnT (Slc30a family) effuses zinc ion from cytoplasm and ZIP (Slc39a family) infuses it into cytoplasm... 

The functionality and molecular integrity of the semisynthetic proteins was explored with the oncogenic G12V K-Ras4B by investigating its ability to induce differentiation of the rat pheochromocytoma PC12 cell line. Under normal... 

The interaction of PC-12 cells, an immortal nervelike cell line derived from rat pheochromocytoma, with polypyrrole thin films doped with poly(styrene sulfonate) dopant has been studied by Shastri et al. [51,52]. Their studies have shown that PC-12 cells interact and differentiate in a more pronounced manner on polypyrrole substrates as compared with tissue culture polystyrene (TCPS) and poly(L-lactic acid) (PLA) controls in the presence of soluble NGF. Using quantitative image analysis techniques, it has been shown that both the number and length of neurites expressed by the cells on polypyrrole substrates are significantly greater... 

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