Phanerochaete chrysosporium cultures

Reed Canary Grass Phalaris arundinacea) was grown in liquid culture and exposed to RDX that was metabolized to the potentially toxic 4-nitro-2,4-diazabutanal (Just and Schnoor 2004). This metabolite is also produced from RDX by strains of Rhodococcus sp. and from the homologous octahydro-l,3,5,7-tetranitro-l,3,5,7-tetrazocine (HMX) by Phanerochaete chrysosporium. 

Choi, S.H., Moon, S.H., and Gu, M.B., Biodegradation of chlorophenols using the cell-free culture broth of Phanerochaete chrysosporium immobilized in polyurethane foam, J. Chem. Technol. Biotechnol., 77, 999-1004, 2002. 

Yu G, Wen X, Li R, Qian Y (2006) In vitro degradation of a reactive azo dye by crude ligninolytic enzymes from nonimmersed liquid culture of Phanerochaete chrysosporium. Process Biochem 41(9) 1987-1993... 

Cell immobilized cultures of Phanerochaete chrysosporium and repeated-batch decolorization were reported by Yang and Yu [46]. Diazo-dye Red 533 was decolorized by PuF immobilized culture, and decolorization efficiency of 80% or higher was achieved within a period of 1 or 2 days. 

Haapala A, Linko S (1993) Production of Phanerochaete chrysosporium lignin peroxidase under various culture conditions. Appl Microbiol Biotechnol 40 494-498... 

Urra J, Sepulveda L, Contreras E, Palma C (2006) Screening of static culture and comparison of batch and continuous culture for the textile dye biological decolorization by Phanerochaete chrysosporium. Brazilian J Chem Eng 23 281-290... 

Initial adsorption of the dyes on fungal biomass followed by degradation was observed in cultures of Irpex lacteus, Phanerochaete chrysosporium, Trametes versicolor, and Trichophyton rubrum [46 -9]. In P. sajor-caju, it was observed that Disperse Blue 79 and Acid Red 315 were incompletely or not degraded, but a decolorization was reached due to adsorption to the mycelium [38]. Also algae can be used as biosorbents of azo dyes [50]. 

CASRN 35065-27-1 molecular formula C12H4CI6 FW 360.88 Biological. Nitrogen-deficient cultures of the white rot fungus Phanerochaete chrysosporium degraded 2,2, 4,4, 5,5 -hexachlorobiphenyl to carbon dioxide (Bumpus et al., 1985). 

Microorganism. All experiments reported here were performed with Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725). Cultures were obtained from the Center for Forest Mycology at the Forest Products Laboratory, Madison, WI, and were maintained as previously described (72). 

Figure 1. 3-0-4 lignin substructure model compounds and products of their degradation by ligninolytic cultures of Phanerochaete chrysosporium and by lignin peroxidase. D H, and 0 of 3-ether bond and of H2 0, respectively. B and C show results of stable isotope experiments. 

Jaspers, C.J. Jiminez, G. Penninck, M.J. Evidence for a role of manganese peroxidase in the decolorization of Kraft pulp bleach plant effluent Phanerochaete chrysosporium effects of initial culture conditions on enzyme production. J. Biotechnol. 1994, 37, 229-234. 

Mougin, C., C. Laugero, M. Asther, and V. Chaplain (1997). Biotransformation of. v-triazine herbicides and related degradation products in liquid cultures by the white rot fungus Phanerochaete chrysosporium. Pestic. Sci., 49 169-177. 

Cornwell RL, Tinland-Butez M-F, Tardone PJ, Cabasso I, Hammel KE. Lignin degradation and lignin peroxidase production in cultures of Phanerochaete chrysosporium immobilized on porous ceramic supports. Enzyme Microb Technol 1990 12 916-920. 

Kuwahara M, Glenn JK, Morgan MA et al (1984) Separation and characterization of two extracelluar H202-dependent oxidases from ligninolytic cultures of Phanerochaete chrysosporium. FEBS Lett 169 247-250... 

Scheme 1. Model compounds are being used to elucidate the specific reactions of lignin decomposition by white-rot fungi. The fate of a nonphenolic dia l-propane-tupe of model in ligninolutic cultures of Phanerochaete chrysosporium Burds is shown (113-15).

Besides LiP, one of the most stndied lignin degrading phenoloxidases is MnP, another heme containing extracellnlar fnngal peroxidase. MnP was hrst identified in ligni-nolytic cultures of Phanerochaete chrysosporium as a Mn(ll) and H2O2 dependent oxidase [55]. Later it was pnrified and characterized from both P. chrysosporium and Trametes versicolor [74-77]. 

TK Kirk, E Schultz, WJ Connors, LF Loren, JG Zeikus. Influence of cultural parameters lignin metabolism by Phanerochaete chrysosporium. Arch Microbiol 117 227-285,1978. 

ID Reid, EE Chao, SS Dawson. Lignin degradation by Phanerochaete chrysosporium in agitated cultures. Can JMicrobiol 31 88-90, 1985. 

A Jager, S Croan, TK Kirk. Prodnction of ligninases and degradation of lignin in agitated submerged cultures of Phanerochaete chrysosporium. Appl Environ Microbiol 50 1274-1278, 1985. 

Isono Y, Nakajima M (2000) Membrane phase separation of aqueous/alcohol biphase mixture and its application for enzyme bioreactor. Prog Biotechnol 16 63-68 Jaspers CJ, Jimenez G, Penninckx MJ (1994) Evidence for a role of manganese peroxidase in the decolorization of Kraft pulp bleach plant effluent by Phanerochaete chrysosporium effects of initial culture conditions on enzyme production. J Biotechnol 37(3) 229-234 Johannes C, Majeherezyk A, Huttermaim A (1996) Degradation of anthracene by laccase ofTram-etes versicolor in the presence of different mediator compounds. Appl Microbiol Biotechnol 46(3) 313-317... 

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