Multiple peptide synthesis

Haack M, Beck-Sickinger AG (2007) Multiple Peptide Synthesis to Identify Bioactive Hormone Structures. 278 243-288... 

W Rapp, E Bayer. Uniform microspheres in peptide synthesis ultrashort cycles and synthesis documentation by on-line monitoring as an alternative to multiple peptide synthesis, in CH Schneider, AN Eberle, eds. Peptides 1992. Proceedings of the 22nd European Peptide Symposium. Escom, Leiden, 1992, pp 25-26. ( magic mixture ). 

G. Jung, A.G. Beck-Sickinger, Multiple peptide synthesis methods and their applications. New synthetic methods (87), Angew. Chem. Int. Ed. 31(4) (1992) 367-486. 

Jung, G. Beck-Sickinger, A. G. Multiple Peptide Synthesis Methods and Their Applications, Agnew. Chem. Int. Ed. Engl. 1992,31, 367. 

In 1985 Houghton introduced his tea bag method for the rapid solid phase multiple peptide synthesis. In this technique the beads are contained within a porous polypropylene bag. All the reactions, including deprotections, are... 

The protocols described here for multiple peptide synthesis can be transferred to any other instrument by correcting the volumes and synthesis scale accordingly without changing the chemistry, reagent excess, etc. 

Lam KS, Lebl M, From multiple peptide synthesis to peptide libraries, in Peptide and Non-Peptide Libraries A Handbook for the Search of Lead Structures (Ed. Jung G), pp. 173-201, 1996, VCH, Weinheim, Germany. 

Houghten, R. A., DeGraw, S. T., Bray, M. K., Hoffmann, S. R., and Frizzell, N. D. (1986) Simultaneous multiple peptide synthesis The rapid preparation of large numbers of discrete peptides for biological, immunological, and methodological studies. BioTechniques 4, 522-528. 

Frank, R. and Coring, R. (1988) Simultaneous multiple peptide synthesis under continuous flow conditions on cellulose paper discs as segmental solid supports. 

Krchnak, V. and Vagner, J. (1990) Color-monitored solid-phase multiple peptide synthesis under low-pressure continuous flow conditions. Peptide Res. 3, 182-193. 

Schnorrenberg, G. and Gerhardt, H. (1989) Fully automatic simultaneous multiple peptide synthesis in micromolar scale Rapid synthesis of series of peptides for screening in biological assays. Tetrahedron 45, 7759-7764. 

Gausepohl, H., Kraft, M., Boulin, C., and Frank, R. W. (1990) A robotic workstation for automated multiple peptide synthesis. In Innovations and Perspectives in Solid Phase Synthesis (Epton, R., ed.), SPCC, Birmingham, UK, pp. 487-490. 

ACT produces the Model 348 Multiple Peptide Synthesizer for researchers who do not require a sophisticated instrument for multiple peptide synthesis. This synthesizer is similar to the Model 396 MBS except it possesses a single robotic arm. 

The Pioneer Peptide Synthesis System has an option of a multiple peptide synthesis (MPS) accessory with a 16-column capacity. A maximum of two MPS units may be added to each Pioneer extending the capabilities from 2 to 32 columns. The MPS unit possesses scales at 25 and 50 pmol compatible with 1.0-mL and 1.6-mL columns. The Fmoc amino acids are predissolved and an 8-fold excess is used. While the DMF washings and 20% piperidine/ DMF deprotections utilize continuous-flow conditions, HATU/DIPEA-mediated couplings incorporate static flow conditions. 

Pioneer Multiple Peptide Synthesis Accessory Users Guide, PerSeptive Biosystems, Inc. Framingham, (1997) p Al. 

Most synthetic methods for the generation of peptide libraries have been derived from various multiple parallel peptide synthesis techniques developed since 1984.bs-i l Consequently, the sohd supports used for hbrary synthesis are essentially the same as those used for multiple peptide synthesis. Standard divinylbenzene cross-linked polystyrene resins are typically used for hbraries that are cleaved from the resin and screened in solution.P Polyoxyethylene-grafted polystyrene resins,f l or acrylamide-polyoxyethylene copolymers, P on the other hand, are the sohd supports of choice for the synthesis of resin-bound peptide hbraries screened in sohd-phase binding assays.P Such resins are compatible with both organic solvents used for peptide synthesis, as well as aqueous buffers used in the bioassays. Various segmental supports previously employed for multiple peptide syntheses have also been utilized for the synthesis of peptide libraries, including polypropylene pins, PI cotton,t cellulose membrane,and glass shdes.P ... 

RA Houghten, ST DeGraw, MK Bray, SR Hoffmann, ND Frizzell. Simultaneous multiple peptide synthesis. Biotechniques 4 522-528, 1986. 

In the so-called tea-bag method, originated in 1984 by Houghten et al. [14] for multiple peptide synthesis, the split-pool protocol occurs batchwise on 15 x 22 mm polypropylene mesh packets with pm-sized pores known as tea bags, sealed with resin beads for solid-phase synthesis. This method offers the advantage that a greater quantity of each compound of the library is available at once (up to 500 pmol), which is sufficient for a complete biological and structural characterization. Furthermore, the structural identity of... 

Gausepohl H, Boulin C, Kraft M, Frank RW (1992) Automated multiple peptide synthesis. Pept Res... 

Beck-Sickinger, A.G., Durr, H., Gaida, W. Jung, G. (1993a) Characterisation of the binding site of neuropeptide Y to the rabbit kidney receptor using multiple peptide synthesis. Biochem. Soc. Trans. 20, 847-850. 

DR Knapp, JE Oatis, DI Papac. Small-scale manual multiple peptide synthesis system. Int J Peptide Protein Res 42 259, 1993. 

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