Pharmacia Biosensor

BIAlite system. Sensor Chip CMS, HBS buffer (lOmM H es, pH 7.4, 150 mM NaCl, 0.005% v/v surfactant p20 in distilled water), amine coupling kit were fi-om Pharmacia Biosensor (Uppsala, Sweden). 

Pharmacia Biosensor AB, Uppsala, Sweden BIAcore Immunologic surface plasmon resonance Biomoiecular interactions... 

Very few immunosensors are commercially available. The commercial immunosensors are either the detector or bioanalyzer types. The PZ 106 immunosensor from Universal Sensors Inc. (New Orleans, LA) has been used as a detector to measure antibody-antigen reaction. Ohmicron (Newtown, PA) developed a series of pesticide immuno-bioanalyzers that have been used in field tests. Pharmacia Biosensor USA (Piscataway, NJ) recently introduced BIAcore immunodetection system. A combination of a unique flow injection device and surface plasmon resonance (SPR) detection technique provides a real time analysis. A carboxylmethyldextran layer added to plasmon generating gold film is a hydrophobic, activatable, and flexible polymer that provides high antibody and low non-specific bindings. System demonstration at the Institute of Food Technologists (IFT) 1994 meeting in Atlanta drew attention of food scientists. It should easily be adapted for food protein characterization. 

Pharmacia Biosensor AB, J. Biomol. Interaction Anal. 1st Edition (1994). 

The surface matrix of a carboxymethylated sensor chip CMS (Pharmacia Biosensor, Uppsala, Sweden) was activated by injection of 35 pL of 0.05 M N-hydroxy-succinimide (NHS)/0.2 M N-ethyl-N (dimethylaminopropyl)carbodiimide (EDC). The BIAcore IFC forms four parallel flow cells on the sensor chip. Three of them were derivatized with 40 mM cystamin-dihydrochloride/ethanolamine in the ratios... 

Protease assay CMV protease activity was measured using the surface plasmon resonance technology of the BIAcore instrument (Pharmacia Biosensor). Briefly, this instrument was used to quantitate residual uncleaved biotinylated... 

Russ Granzow and Osten Jansson Pharmacia Biosensor AB, S-751 82 Uppsala, Sweden... 

Perkin-Elmer Corporation, Applied Biosystems Division PerSeptive Biosystems, Inc. Pharmacia Biosensor Pharmacia Biotech, Inc. 

The first commercial SPR was launched hy Pharmacia Biosensor AB (presently Swedish BIAcore AB) in 1990. Since then, the device has been refined and now BIAcore [37] offers several models (BIACORE 3000, BIACORE 2000, BIACORE 1000, BIACORE X, J, Q, S51, and C models). The biosensors of BIACORE 1000 to 3000 are fully automated instruments, with a disposable sensor chip, an optical detection unit, an integrated micro-fiuidic cartridge, an autosampler, method programming and control software. Less expensive manually controlled alternatives cU e the BIACORE x and BiacoreQuant . 

FIA as such is available from a few different suppliers. Reactors filled with immobilized enzymes are also commercially available. More sophisticated equipment based on the FIA concept is the BIACORE from Pharmacia Biosensor. Here a gold surface covered with a carboxydextran is used as reactor for binding reactions. The reading is done by means of evanescent wave technology [63]. The BIACORE illustrates one step in the direction of miniaturization of the analytical unit. As the understanding of the chemistry and the analytical protocol becomes available, much may be gained by miniaturization [64]. 

Pharmacia Biosensor AB Multiple antigens Antibody-coated surface plas-... 

This optical design has been further advanced by Biacore (Pharmacia Biosensors AB since 1996, Biacore AB) and resulted in a family of commercial SPR sensors [20-22] offering high performance (resolution down to 1x10 RIU) and multiple sensing channels (up to four) for simultaneous measurements. 

In 1982 researchers from Pharmacia started to work jointly with physics and biochemistry professors at Linkoping University in order to devebp a new bioanalytical instrument able to mcKiitor the interactions between biomolecules. In 1984 a new company Pharmacia Biosensor was created. 

Pharmacia Biosensor AB Label-free surface plasmon resonance (SPR) based technology for studying biomolecular interactions in real time. 

The SPR-based biosensor systems BlAcore and BlAlite. developed by Pharmacia Biosensor AB and now available commercially, represent a breakthrough in immunosensor technology, particularly in the use of SPR techniques. Apart from high cost, the only limitation associated with this technique is that the sensitivity depends on the... 

In essence, this technique allows for the detection of surface binding interactions in real time without the use of labels. Immunosensors that rely on these principles are commercially available from Biacore AB (formerly Pharmacia Biosensor). These are based on the popular BIACORE 3000, BIACORE 2000 and BIACORE probe instruments in conjunction with disposable sensor chips, which may be purchased with affinity capture chemistries in place (e.g. SA5 Sensor Chips with streptavidin bearing surfaces for capture of biotinylated compounds) [92]. These devices incorporate micro-fluidic systems for delivery of reagents required for analysis and are capable of measuring affinities and kinetics of antibody-antigen interactions in addition to analyte concentrations, which can be determined to the picomolar range [93]. 

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