Patch clamp recordings

Figure 13.3 Whole-cell patch-clamp recordings of excitatory postsynaptic currents (EPSCs) from dorsal horn neurons of rat (prenatal P2-13) spinal cord slices. The normal evoked EPSC of about 160pA obtained by focal stimulation of nearby tissue was dramatically reduced by addition of a cocktail (CABS) of CNQX 10 pM, D-APV 50 pM, bicuculline 10 pM and strychnine 5 pM to block glutamate, GABAa and glycine receptors. The small residual EPSC shown was blocked by the ATP P2 receptor antagonist suramin and is therefore probably mediated by released ATP. (Prom Bardoni et al. 1997 and reproduced by permission of the Journal of Neuroscience)...

Trotier D., Dpving K., Ore K. and Shalchian-Tabrizi C. (1998). Scanning electron microscopy and gramicidin patch clamp recordings of microvillous receptor neurons dissociated from the rat vomeronasal organ. Chem Senses 23, 49-57. 

Selected entries from Methods in Enzymology [vol, page(s)] Analysis, software for, 207, 717 barrier models, 207, 818 closed and open time estimation, 207, 755 data acquisition, 207, 747 modal behavior analysis, 207, 757 multiple channel problem, 207, 756 single-channel [extraction of kinetic information, 207, 765 measurement in tissue slices, 207, 220] synaptic, resolution improvement in patch clamp recording, 207, 216 whole-cell recording in calcium channel, 207, 181 fluctuation analysis, 207, 192. 

A patch-clamp (recording) pipette is attached to an individual cell and a gigaohm seal is formed between the cell and the patch-clamp pipette by applying suction through the pipette. 

Cui, C., Smith, D.O. and Adler, J. (1995) Characterization of mechanosensitive channels in Eschericia cofi cytoplasmic cell membrane by whole-cell patch clamp recording. Journal of Membrane Biology 144 31-42... 

This variability in temporal characteristics also exists across insect species. For instance, in cockroaches certain ORNs show onset and offset of firing correlated with stimulation (Getz and Akers, 1997) whereas some ORNs in T sensilla of moths continue firing after the end of odor delivery (Anderson et al., 1995). The occurrence of excitatory and inhibitory responses in single ORNs (depolarization and hyperpolarization, respectively) was noted by Boeckh (1967) in a beetle and a blowfly and is well documented in lobsters (Ache and Zhainazarov, 1995). Inhibitory responses were also observed in patch-clamp recordings of Drosophila antennal ORNs (Dubin and Harris, 1997). Hyperpolarizing currents were due to a decrease in an outward Cl current. 

The eag potassium channel can be modulated by cyclic nucleotides and may play a role in ORNs. Patch-clamp recordings from antennal ORNs in eag mutants show fewer neurons responding to ethyl butyrate (Dubin et al., 1998). Application of high K+ saline to the dendritic side of ORNs raises excitability of most wild type neurons but not in eag mutants, suggesting a role for eag in transduction in ORN dendrites rather than during development (Dubin et al., 1998). 

Cepeda C, Chandler SH, Shumate LW, Levine MS (1995) Persistent Na+ conductance in medium-sized neostriatal neurons characterization using infrared videomicroscopy and whole cell patch-clamp recordings. J Neurophysiol 74 1343—1348. 

Figure 5. The four-patch clamp recording methods. In each example, the cell is shown to the left, the micropipette to the right. For a discussion of the methods see text. Reproduced with permission from Hille, B., "Ionic channels of excitable membranes" 1 st ed., Sinauer, Sunderland, MA (1984) p. 217.

HamiU OP, McBride DW. Induced membrane hypo/hyper-mechanosensitivity A limitation of patch-clamp recording. Annu. 34. Rev. Physiol. 1997 59 621-631. 

Fertig N, Blick RH, Behrends JC. Whole cell patch clamp recording performed on a planar glass chip. Biophys. J. 2002 82 3056-3062. 

Fignre 13.16. Patch-Clamp Recordings of the Acetylcholine Receptor Channel. Patch-clamp recordings illustrate changes in the conductance of an acetylcholine receptor channel in the presence of acetylcholine. The channel undergoes frequent transitions between open and closed states. [Courtesy of Dr. D. Colquhoun and Dr. B. Sakmann.]... 

Hardy A, Ptdouzier-Paulignan B, Duchamp A, Royet JP, Duchamp-Viret P. 2005. 5-hydroxytryptamine action in the rat olfactory bulb In vitro electrophysiological patch-clamp recordings of juxtaglomerular and mitral cells. Neuroscience 131 717-731. 

Espinosa, F., de la Vega-Beltran, J.L., Lopez-Gonztilez, I., Delgado, R., Labarca, P., and Darszon, A. (1998). Mouse sperm patch-clamp recordings reveal single CL channels sensitive to niflumic acid, a blocker of the sperm acrosome reaction. FEBS Lett. 426 47- 51. 

Figure 2. A. Recording of calcium variations in a photocyte cluster of O. aranea. (i) 200mM KCl stimulation, ( (>) rinsing with ASW. Values are expressed as a ratio between Ca bound fura-2 and free fura-2. B. Whole cell patch-clamp recording on O. californica photocyte. Stimulation protocol holding potential of-lOOmV, steps of 25mV from -lOOmV to -i-lOOmV (a) outward current, (b) inward current...

Hausser M (1994) Kinetics of excitatory postsynaptic currents in Purkinje cells studied using dendritic patch-clamp recording. Soc. Neurosci. Abstr. 20 372.7. 

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