Parallel genetic analysis

A better method for highly parallel genetic analysis is needed. One with single molecule sensitivity that eliminates both the requirement for target amplification and the need for a target to be chemically modified or labeled for its detection would be ideal. Innovations in array construction, sample nucleic acid preparation... 

Current developments are not only aimed at integration but also at parallel genetic analysis as well. Parallel genetic analysis allows detection of different DNA samples simultaneously. A microfluidic device integrated with multichamber PCR and multichannel CE for parallel genetic analysis was developed (Figure 10.4) [56]. The microdevice consists of three functional units temperature control, multiple PCR (four chambers PCR), and multiple channel separation (four separation channels, each channel cormected to a PCR chamber). A separation channel is... 

Lashkari, D.A., DeRisi, J.L., McCusker, J.H., Namath, A.F., Gentile, C., Hwang, S.Y., Brown, P.O., and Davis RW, Yeast microarrays for genome wide parallel genetic and gene expression analysis, Proc. Natl. Acad. Sci. USA, 94, 13507-13062, 1997. 

Lashkari DA, DeRisi XL, McCusker JH et al. Yeast microarrays for genome-wide parallel genetic and gene expression analysis. Proc Natl Acad Sci USA 1997 94 13057-13062. 

The microfluidic chip uniquely facilitates high-throughput genetic analysis. For instance, 12 different samples were analyzed in parallel by capillary array electrophoresis (CAE) see Figure 9.20. The two-color method was used for... 

Cho, R.J., Fromont-Racine, M., Wodicka, L., Feierbach, B., Stearns, T., Legrain, P., Lockhart, D.J., and Davis, R.W. (1998b) Parallel analysis of genetic selections using whole genome oligonucleotide arrays. Proc Natl Acad Sci USA 95 3752—3757. 

Paolocci F, Robbins MP, Madeo L, Arcioni S, Martens S, Damiani F. 2007. Ectopic expression of a basic helix-loop-helix gene transactivates parallel pathways of proanthocyanidin biosynthesis. Structure, expression, analysis, and genetic control of leucoanthocyanidin 4-reductase and anthocyanidin reductase genes in Lotus corniculatus. Plant Physiol 143 504-516. 

The yield of the expected reaction product was used in an example as the feedback to a genetic algorithm (GA) driven method that proposes a new set of reaction conditions. After some cycles of synthesis and analysis the yield of this reaction was significantly improved by using better reaction conditions. In a second step, a set of different MCRs using a set of different conditions for each of them was carried out in parallel and optimized with a GA to find common optimal conditions [29]. 

DD Shoemaker, DA Lashkari, D Morris, M Mittmann, RW Davis. Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular barcoding strategy. Nature Genet 14 450-456, 1996. 

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