Oxidase enzymes chemical

Several biosensors are commercially available. One of the most useful is the glucose sensor. The standard sensor determines glucose concentration based on the glucose oxidase enzyme. The chemical reaction for oxidation of glucose is ... 

Acetylcholineesterase and choline oxidase Enzymes were co-immobilized on chemically preactivated immun-dyne polyamide membrane (thickness 120 pm, size cut-off 3 pm). Applying 20 pL of 1% ChO solution in 0.1 M-phosphate buffer (pH 6). Assay was based on electrochemical detection of the generated h2o2. The response time was 2min. Detection limit was 50 nM and the response was rectilinear up to 20 pM. [71]... 

Cholesterol can be determined by immobilizing the enzyme cholesterol oxidase (COD) in a layer over an oxygen electrode or a hydrogen peroxide electrode. Cholesterol oxidase is chemically immobilized onto nylon net (135, 265) or collagen membranes (267) and fixed onto an O2 electrode ... 

ADP AFP ab as ALAT AP ASAT ATP BQ BSA CEH CK CME COD con A CV d D E E EC ECME EDTA EIA /e FAD FET FIA G GOD G6P-DH HBg HCG adenosine diphosphate a-fetoprotein antibody antigen alanine aminotranferase alkaline phosphatase aspartate aminotransferase adenosine triphosphate benzoquinone bovine serum albumin cholesterol ester hydrolase creatine kinase chemically modified electrode cholesterol oxidase concanavalin A coefficient of variation (relative standard deviation) layer thickness diffusion coefficient enzyme potential Enzyme Classification enzyme-chemically modified electrode ethylene diamine tetraacetic acid enzyme immunoassay enzyme loading factor flavin adenine dinucleotide field effect transistor flow injection analysis amplification factor glucose oxidase glucose-6-phosphate dehydrogenase hepatitis B surface antigen human chorionic gonadotropin... 

T. C. Bruice, Chemical Studies and the Mechanism of Flavin Mixed Function Oxidase Enzymes (R), in J. F. Liebman and A. Greenberg, eds.. Mechanistic Principles of Enzyme Activity, VCH Publishers,... 

Enzymes are often employed in the chemical layer to impart the selectivity needed. We saw an example of this in Chapter 13 when discussing potentiometric enzyme electrodes. An example of an amperometric enzyme electrode is the glucose electrode, illustrated in Figure 15.4. The enzyme glucose oxidase is immobilized in a gel (e.g., acrylamide) and coated on the surface of a platinum wire cathode. The gel also contains a chloride salt and makes contact with silver-silver chloride ring to complete the electrochemical cell. Glucose oxidase enzyme catalyzes the aerobic oxidation of glucose as follows ... 

The diphenyl ether nitrofen (1) [24], introduced in 1963 by Rohm and Haas, now Dow AgroSciences the oxadiazolinone oxadiazon (2) [25, 26] (Explorer , Herbstar , Romax , Ronstar ), introduced in 1968 by Rhone-Poulenc and the tetrahydrophthalimide chlorophthalim 3 [27], introduced in 1972 by Mitsubishi, represent the earliest examples of Protox herbicides (Fig. 3.2). Though all three classes are chemically quite different, they share a common mode of action, inhibition of the protoporphyrinogen oxidase enzyme, though this was not known until the late 1980s. 

In 1988, a pioneering work dealing with electrode characteristics of an immobilized enzyme chemically modified electrode based on bilayer-film coating for amperometric determination of glucose was reported. In this work, a substrate was coated with two kinds of polymeric films in a bilayer state, that is, first with the cobalt tetrakis(o-aminophenyl)porphyrin polymer film and then with the enzyme film consisting of bovine serum albumin and glucose oxidase that were... 

Jiang, H. Ju, H.X. (2007). Enzyme-quantum dots architecture for highly sensitive electrochemiluminescenece biosensing of oxidase substrates. Chemical Communications, 4,404-406. 

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