Collagen Membranes

VIII. Scleroproteins, e.g., keratin from hair, horn, feathers, egg-membrane. Collagen, gelatin, elastin. Silk-fibroin, silk-gelatin,... 

Brazel, D., Pollner, R., Oberbaumer, I., and Kuhn, K. (1988). Human basement membrane collagen Type IV The amino acid sequence of the a2(IV) chain and... 

Sado, Y., Kagawa, M., Naito, I., Ueki, Y., Seki, T., Momota, R., Oohashi, T., and Ninomiya, Y. (1998). Organization and expression of basement membrane collagen IV genes and their roles in human disorders./. Biochem. (Tokyo) 123, 767-776. 

Yurchenco, P. D., and Furthmayr, H. (1984). Self-assembly of basement membrane collagen. Biochemistry 23, 1839-1850. 

Preparation of Collagen Membranes. Collagen dispersion was prepared from hide collagen by dispersing freeze-dried collagen in aqueous lactic acid solution at pH 2.8. The collagen dispersion was cast to form a membrane (17). The thickness of the dried membrane was between 0.028 and 0.033 mm. 

Preparation of Chemically Modified Collagen Membranes. Collagen membrane was prepared by the above procedure. The lysyl E-amino residues of collagen, in membrane form, were modified to varying degree by reaction with potassium cyanate. The collagen membrane was carbamylated by immersing preswollen films in 0.4M potassium cyanate solution, pH 8.5 for different periods of time. The carbamylation reaction was carried out at ambient temperature (approx. 20°C). The pH of the reaction mixture was monitored with a pH meter and the pH maintained at 8.5 by the addition of 0.05N... 

Unless otherwise stated, the enzyme binding capacity of membraneous collagen was measured by the equilibrium sorption method. 

Chemical Modification of Collagen- The Effect of Carbamyla-tion on the Enzyme-Binding Capacity of Membraneous Collagen. 

B. Effect of Carbamylation on Enzyme Binding. Figure 1. shows the effectiveness of chemical modification (cambamylation) in reducing the enzyme binding activity of membraneous collagen, where the quantity of enzyme sorbed is plotted as a function of... 

Lauer-Fields JL, Malkar NB, Richet G, Drauz K, Fields GB. Melanoma cell CD44 interaction with the alpha 1 (TV)1263-1277 region from basement membrane collagen is modulated by ligand glycosylation. J. Biol. Chem. 2003 278 14321-14330. 

Collier, I. E. Wilhelm, S. M., Eisen, A. Z., Marmer, D. L., Grant, G. A., Seltzer, J. L., Cronberger, A., He, C., Bauer, G. A. and Goldberg, G. I. (1988). H-ras-oncogene-transformed human bronchiolar epithelial cells (TBE-1). secrete a single metalloproteinase capable of degrading basement membrane collagen. J. Biol. Chem. 263, 6579-6587. 

Liotta, L. A., Tryggvason, K., Garbisa, S., Hart, I., Foltz, C. M. and Shafie, S. (1980a) Metastatic potential correlates with enzymatic degradation of basement membrane collagen. Nature 284, 67-68. 

E. A. and Goldberg, G.A. (1988). H-nw oncogene-transformed human bronchial epithelial cells (TBE-1) secrete a single metalloproteinase capable of degrading basement membrane collagen. J. Biol. Chem. 263, 6579-6587. 

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