Ethylene diamine tetraacetic acid

Gardiner [84,85] described a gas chromatographic method for the determination of EDTA in aqueous environmental samples. The separation of the major peaks is increased by preparing the ethyl derivatives of the sample compounds, 1,6-hexanediamine tetraacetic acid (HDTA) being used as internal standard. The lower limit of detection of the method is approximately 15pg Lwith 25mL samples. In this method the ethyl derivatives of the sample components were prepared so that the major peaks would be well separated. The ethyl esters of fatty acids up to and including the C,8 fatty acids eluted well before the EDTA derivatives and did not interfere. 

Kari [86] and Schiirch and Dubendorfer [87] both point out that gas chromatographic procedures need lengthy sample pretreatments. 

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Dehydrogenation of the hydrazide derivative 33 with mercuric oxide in the presence of ethylene diamine tetraacetic acid (EDTA) gave 34 and 35 (77AP588). The latter (35) was prepared from a reaction of ester 36 with the appropriate lactam 37 (Scheme 11). 

In this laboratory, we also include the metal ion chelators EDTA (ethylene diamine tetraacetic acid binds, e.g., Mg2 1 -ions) and EGTA (ethylene glycol-bis(2-aminoethyl)-Al,iV,iV/,iV/,-tetraacetic acid binds, e.g., Ca2+-ions) in our lysis buffers. These agents help prevent phosphatase action (by the metal ion-dependent phosphatase PP2C, which is not inhibited by microcystin-LR), metal (Ca2+) dependent proteinases, and protein kinases, which require divalent cations such as Mg2 1 (and, in some cases, also Ca2+). We also use a mix of proteinase inhibitors that inhibit a broad range of proteolytic enzymes, including serine and cysteine proteinases. 

Fe(IIJ) (S) 1 -(p-bromoacetamido-benzyl) ethylene diamine tetraacetic acid MW 589.15... 

Epidermis Complete removal of the dermis may be achieved by several mechanical, thermal, and chemical techniques. Most commonly, the epidermal-dermal junction is split by heating the skin to 60 C for 30-120 s [83, 84], Pitman et al. [85] could show that such a treatment does not impair the barrier function. The use of ethylene diamine tetraacetic acid, sodium bromide, or ammonia fumes has also been reported [80, 83, 86], It may, however, be suspected that the use of sufficiently strong acids or bases may change the buffer capacity of skin, which would especially influence the penetration behavior of ionizable drugs. 

The (8O3) radical is essentially the sulfur trioxide anion-radical. It is named the sulfite anion-radical because it is obtained from sodimn sulfite on reaction with titanimn trichloride in water. This reaction usually proceeds in the presence of ethylene diamine tetraacetic acid as a complexing agent and hydrogen peroxide as an oxidant (Bradic and Wilkins 1984). Under these conditions, the... 

APHA = American Public Health Association EDTA = ethylene diamine tetraacetic acid EPA = Environmental Protection Agency Kl = potassium iodide OSHA = Occupational Safety and Health Administration UVA/IS = ultravioletA/isible light... 

Laboratory research has demonstrated that the liquid corona technology can treat a variety of wastewater contaminants such as carbon tetrachloride, metal ion chelators, and industrial dyes. The technology successfully reduced initial organic contaminant concentrations (by more than 99%) for the following contaminants after exposure to corona discharge trichloroethylene (TCE), ethylene-diamine-tetraacetic acid (EDTA), and benzoic acid. Additionally, liquid corona has demonstrated removal success with carbon tetrachloride, pentachlorophenol, and perchloroethylene. 

The effect of ligands on the character and degree of the inner-sphere reorganization during electroreduction of aqua-, aquahydroxy-, hydroxy-, and ethylene-diamine tetraacetic acid (EDTA) complexes of Zn(II) [95] and electrochemical process of Zn(II) complexed by different ligands - glycinate [96], ethanol amine [97], azinyl methyl ketoximes [98], aspartame [99], glutathione [100, 101] and several cephalosporin antibiotics [102] -were studied at mercury electrodes in aqueous solutions. 

The sulfite anion radical (SOI) is obtained from sodium sulfite upon the action of titanium trichloride in water in the presence of ethylene diamine tetraacetic acid (as a complexing agent) and hydrogen peroxide (Bradic Wilkins 1984). Scheme 1-91 below shows this type of SOI generation. 

Microcystin-LR (MC-LR) (Sigma, France) standard solutions first prepared in 50 50 methanokwater and subsequently diluted in 30 mM tris-HCl, 2mM dithiothreitol (DTT), 2mM ethylene diamine tetraacetic acid (EDTA), 0.2mgmL 1 bovine serum albumin (BSA), 20 mM MgCl2 buffer (pH 8.4) (prepared in Milli-Q water). 

An extraction procedure based on ethylene diamine tetraacetic acid has been evaluated for the extraction of metals from soils [26]. In a collaborative study between 54 different laboratories, all of the laboratories produced some extreme outlying results, but most results were in good agreement once the outliers had been removed. 

Vickery and Vickery [9] have investigated the interference by aluminium and iron in the ion-selective electrode method for the determination of fluoride in plant extracts. They demonstrated that plant ashes may contain sufficient of these two elements to seriously interfere in the determination of fluoride when using the fluoride-selective electrode. In the presence of these metals, the known additions method gives erroneous results, as did that involving the attempted formation of complexes with ethylene diamine tetraacetic acid (disodium salt) or 1,2-cyclohexylenedinitrilotetraacetic acid. 

Prepare the antibody for reduction. For labeling IgG antibodies with thiol-reactive reagents, the antibody should be at 5-10 mg of pro-tein/mL in 20 mM sodium or potassium phosphate buffer, pH 7.5-8.0 containing 150 mill NaCl or 10 mM phosphate-buffered saline ethylene diamine tetraacetic acid (PBS-EDTA). When biotin maleimide or biocytin maleimide derivatives are used, the buffer used for the reduction and subsequent steps should be at pH 6.5-7.2. 

X TBE buffer for agarose gel electrophoresis Trizma base, 27 g boric acid, 13.75 g ethylene diamine tetraacetic acid, 2.33 g. Make up to 100 mL. 

Labeling buffer PBS supplemented with 0.5% bovine serum albumin and 2 mM ethylene diamine tetraacetic acid. 

Add 5 mM ethylene diamine tetraacetic acid to the sample then add 0.82 vol of saturated ammonium sulfate solution while stirring and leave for 15 min at room temperature. 

Inspect the cells on d 3, they should be almost fully confluent on the flask base. Discard the medium, add 10 mL of cold sterile PBS containing 0.02% ethylene diamine tetraacetic acid and leave for a few minutes to allow the cells to detach. Split the cells equally between two flasks and add 75 mL of RPMI 1640 medium/5% FBS to each flask and return to the incubator. 

On d 4, remove the medium from each flask and replaced with 20 mL of cold sterile PBS containing 0.02% ethylene diamine tetraacetic acid. After a few minutes the cells will detach from the flask surface. Harvest the cells by gently tapping the flask and pouring the cell suspension in to sterile universal containers. Wash the cells by centrifugation at 300g and resuspend in 10 mL of cold PBS. 

Degassed solution of phosphate-buffered saline (PBS) without calcium and magnesium, supplemented with 0.1% (w/v) bovine serum albumin and 2 mM ethylene diamine tetraacetic acid. 

Lysis buffer 154 mM NH4C1, 10 mM KHC03, 0.1 mM ethylene diamine tetraacetic acid. 

Ethylene diamine tetraacetic acid (EDTA Invitrogen Corporation). 

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