Ononis

The diuretic and cholagog effect of Ononis arvensis motivated the development of a new two-dimensional paper chromatographic and TLC method for the measurement of onion in its roots and aerial parts. Samples were dried, ground and extracted with methanol (70 per cent, w/v) for 2 h. The supernatant was diluted and used for TLC separation on a cellulose stationary phase. The first eluent was 3 per cent formic acid, and the second was n-butanol-acetic acid-water (4 1 5, v/v) for both TLC and paper chromatography. Spots were scraped off, extracted with methanol and the absorption was measued at 260 nm. It was found that the average ononin content in roots and aerial parts was 0.153 0.0278 (per cent) and 0.498 0.045 (per cent), respectively. Because of the simplicity, the method was... 

J. Spilkova, J. Dusek, P. Solich, J. Stranska and K. Ruzickova, Application of two-dimensional chromatography for determination of ononin in the roots and aerial parts of Ononis arvensis L./. Plan. Chromatogr.—Mod TLC. 9 (1996) 299-302. 

Astragalus frigidus Trifolium Ononis repens Vida sativa ... 

Figure 101. RF of QAs individuals in Coronilla varia, Cytisus scoparius, Lotus corniculatus, Lupinus polyphyllus, Meliotus officinalis, Ononis repens, Ornithopus petpusillus, Oxytropis campestris during 1999-2003.

Coronilla varia (1) O Cytisus scoparius (2) T Lotus corniculatus (3) V Lupinus polyphyllus (4) Meliotus officinalis (5) O Ononis repens (6) Ornithopus perpusillus (7) O Oxytropis campestris (8)... 

Ononis repens - Oxytropis campestris - Coroniiia varia - Lotus cornicuiatus D 0.637 ... 

Ononis repens "" - Ornithopus perpusiiius "" - Oxytropis campestris" " - Coroniiia varia""" - Cytisus scoparius""- Lotus cornicuiatus""" F 0.696 ... 

Ononis repens"""- Ornitbhopusperpusiiius""" - Oxytropis campestris"""- Coroniiia varia"""- Cytisus scoparius" - Lotus cornicuiatus""" - Lupinus poiyphyiius G 0.706 ... 

Figure 108. MEC of Coronilla, Cytisus, Lotus, Lupinus, Meliotus, Ononis, Ornithopus, Oxytropis spp.

Shaker, K.H. et al., A new triterpenoid saponin from Ononis spinosa and two new flavonoid glycosides from Ononis vaginalis, Z. Naturforsch. B, 59, 124, 2004. 

Wollenweber, E. et al., Externally accumulated flavonoids in three Mediterranean Ononis species, Z. Naturforsch., 58c, 111, 2003. 

Barrero, A.E., Cabrera, E., and Garcia, I.R., Pterocarpans from Ononis viscosa subsp. breviflora. Phytochemistry, 48, 187, 1998. 

Anacardium occidentale L. (Anacardiaceae) [1] Schinus terebinthifolius Raddi (Anacardiaceae) [2] Gingko biloba L. (gingkoaceae) [3] Mangifera indica L. (Anacardiaceae) [4] Ononis speciosa lag (Leguminosae) [5]... 

Eutaxia Burtonia Goodia 2 Oaviesia Lens Vida PIsum Lathyrus Medicago Ononis Cicer Abrus3 Calophaca Caragana Halimodendron Swainsonia... 

Burtcxiia Goodia 2 Daviesia I Lens I Vicia I Pisum I Lalhyrus I Medicago Ononis I Cicer... 

For resorcinolic lipids, particularly those with long saturated side-chains, the use of polar solvents is important due to their amphiphilicity. The crude extracts in many cases are subjected to preliminary fractionation/purification either by solvent fractionation/partition or by application of chromatography. For prepurification of the material and its separation from polymerized phenolics, gel filtration on hydrophobic Sephadex or TSK gel is sometimes used. Silica gel is most frequently employed for the separation and/or purification of resorcinolic lipids, notably in some studies with Ononis species (12-14). The array of compounds reported appears partly attributable to methylation or acetylation reactions occurring during column chromatographic separation. An interesting approach for I the pre-purification and selective separation of resorcinolic lipid from phenolic lipids or resorcinolic lipids from impurities has recently been reported. A selective partitioning of different non-isoprenoid phenolic lipids... 

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