Oligosaccharides capillary electrophoresis

To allow all culture productiou to be coutrolled, a method for rapid analysis is required. Prior to development of an LC-MS method, the analysis was both complex and time-consuming, involving the purification of a relatively large amount of the antibody using affinity chromatography, enzymatic release, and subsequent derivatizafion of the oligosaccharides and their analysis by using capillary electrophoresis. 

Suzuki, S., Kakehi, K., and Honda, S., Two-dimensional mapping of N-gly-cosidically linked asialo-oligosaccharides from glycoproteins as reductively pyridylaminated derivatives using dual separation modes of high-performance capillary electrophoresis, Anal. Biochem., 205, 227, 1992. 

Camilleri, P., Harland, G. B., and Okafo, G., High resolution and rapid analysis of branched oligosaccharides by capillary electrophoresis, Anal. Biochem., 230, 115,1995. 

Guttman, A., Cooke, N., and Star, C. M., Capillary electrophoresis separation of oligosaccharides. I. Effect of operational variables, Electrophoresis, 15,1518, 1994. 

Pathogen-suppressing oligosaccharides in human milk were quantitated using capillary Electrophoresis by MEKC with detection by absorption at 205 nm.90 A 24 kDa glycoprotein associated with muscle-wasting cachexia... 

Shen, Z., Warren, C.D., and Newburg, D.S., High-performance capillary electrophoresis of sialylated oligosaccharides of human milk, Anal. Biochem., 279, 37, 2000. 

Kakehi, K., Kinoshita, M., and Nakano, M. 2002. Analysis of glycoproteins and the oligosaccharides thereof by high performance capillary electrophoresis - significance in regulatory studies on biopharmaceutical products. Biomedical Chromatography 16(2), 103-115. 

B. Lagane, M. Treilhou and F. Couderc, Capillary electrophoresis theory, teaching approach and separation of oligosaccharides using indirect UV detection. Biochem. Mol. Biol. Educ. 28 (2000) 251-255. 

Zamfir A, Seidler DC, Kresse H, Peter-Katalinic J. 2002. Structural characterization of chondroitin/dermatin sulfate oligosaccharides from bovine aorta by capillary electrophoresis and electrospray ionization quadrupole time-of-flight tandem mass spectrometry. Rapid Commun Mass Spectrom 16 2015. 

Taverna, M., Baillet, A., Biou, D., Schluter, M., Werner, R., and Terrier, D. (1992). Analysis of carbohydrate-mediated heterogeneity and characterization of N-linked oligosaccharides of glycoproteins by high-performance capillary electrophoresis. Electrophoresis 13, 359—366. 

Suzuki, H., Muller, O., Guttman, A., and Karger, B. L. (1997). Analysis of 1-aminopyrene 3,6,8-trisulfonate-derivatized oligosaccharides by capillary electrophoresis with matrix- assisted laser desorptlon/ionization tlme-of-fhght mass spectrometry. Anal. Chem. 69, 4554—4559. 

A D Hulst, N Verbecke. Chiral separation by capillary electrophoresis with oligosaccharides. J Chromatogr 608 275-287, 1992. 

H Soini, M Stefansson, M-L Riekkola, MV Novotny. Malto-oligosaccharides as chiral selectors for the separation of pharmaceuticals by capillary electrophoresis. Anal Chem 66 3477-3484, 1994. 

A D Hulst, N Verbeke. Chiral analysis of basic drugs by oligosaccharide-mediated capillary electrophoresis. J Chromatogr A 735 283—293, 1996. 

Mao W, Thanawiroon C, Linhardt RJ (2002) Capillary electrophoresis for the analysis of glycosaminoglycans and glycosaminoglycan-derived oligosaccharides. Biomed Chromatogr 16 77-94... 

Complex carbohydrates released from glycoproteins were readily profiled by capillary gel electrophoresis with LIF-based detection of 1-aminopyrene-3,6,8-trisulfonic acid (APTS)-labeled sugar molecules (Figure 10) [125]. High-mannose-type oligosaccharides of ribonuclease B were derivatized by APTS and separated by capillary electrophoresis using polyethylene oxide separation medium [126]. 

A. G. Gongalves, D. R. B. Ducatti, R. G. Paranha, M. E. R. Duarte, and M. D. Noseda, Positional isomers of sulfated oligosaccharides obtained from agarans and carrageenans Preparation and capillary electrophoresis separation, Carbohydr. Res., 340 (2005) 2123-2134. 

Guerrini M, Raman R, Venkataraman G, Torri G, Sasisekharan R, Casu B. A novel computational approach to integrate NMR spectroscopy and capillary electrophoresis for strucsture assignment of heparin and heparan sulfate oligosaccharides. Glycobiology 2002 12 713-719. 

Carbohydrates play an important role in many research and industrial domains. The huge number of stereoisomers, the immense combination possibilities of carbohydrate monomers in oligosaccharides, and the lack of chromophores are the major problems in the analysis of carbohydrates. Capillary electrophoresis (CE), in its various modes of operation, has been developed as a very useful tool in the analysis of carbohydrate species such as monosaccharides and oligosaccharides, glycoproteins, and glycopeptides. 

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