Malto-oligosaccharides

Cello- and malto-oligosaccharides up to nonasaccharides in the presence of various metal salts have been analyzed by f.a.b.-m.s. The structures of two methyl alduronates obtained by flash hydrolysis of wood chips was deduced by using f.a.b.-m.s., n.m.r. spectroscopy, and sugar analysis. ... 

Hydrophilic interaction chromatography on Asahipak NH2P or Excel-pak CHA-P44 with pulsed amperometric detection has been used to fractionate malto-oligosaccharides.266 The Asahipak NH2P is a polyvinyl alcohol support with a polyamine bonded phase, and the Excelpak is a sulfonated polystyrene in the Zn+2 form. Amine adsorption of sialic acid-containing oligosaccharides was performed on a Micropak AX-5 column (Varian) using acetonitrile-water-acetic acid-triethylamine.267... 

Hotchkiss, Jr., A. T., Haines, R. M., and Hicks, K. B., Improved gram-quantity isolation of malto-oligosaccharides by preparative HPLC, Curb. Res., 242, 1, 1993. 

Malto-oligosaccharide aldonolactones react with ethylenediamine to give Ar-(2-aminoethyl)aldonamides (113-115), which have been successfully grafted onto carriers via amide linkages. The malto-oligosaccharides were produced by degradation of amylose with alpha amylase. After purification of the oligosaccharides, they were converted into the lactones by hypoiodite or electrolytic oxidation. 

The thermodynamics of the maltose-binding protein of E. coli (MalE) have been studied using both DSC and ITC.117 This protein is a periplasmic component of the transport system for malto-oligosaccharides and is used widely as a carrier... 

Coates, M.L. Wilkins, C.L. LD-FT-MS of Malto-Oligosaccharides. Biomed. Mass Spectrom. 1985,12,424-428. 

H Soini, M Stefansson, M-L Riekkola, MV Novotny. Malto-oligosaccharides as chiral selectors for the separation of pharmaceuticals by capillary electrophoresis. Anal Chem 66 3477-3484, 1994. 

Trimethylsilylation is adversely affected by moisture, and therefore, hydrolyzates should be evaporated to dryness as completely as possible. If trimethylsilylation is catalyzed by trifluoroacetic acid, instead of chlorotrimethylsilane, moderate proportions of water may be tolerated,117,127-129 but, even under these conditions, extra peaks may be obtained from partly trimethylsilylated derivatives.130 Catalysis with trifluoroacetic acid is useful when aqueous aliquots from a reaction are to be trimethylsilylated.131 A further advantage of this method, which has been used in the determination of 1,6-anhydro-jS-D-glucopyranose in corn syrup,132 for cycloamyloses,133 and for a series of malto-oligosaccharides,134 is that ammonium trifluoroacetate is soluble in pyridine. 

Fig. 2. Retentions of orange oil after spray drying with wheat, cassava, rice, amylomaize, corn, waxy corn, and potato malto-oligosaccharides.

Figure 4. Photograph of a radioautogram of products from labeled malto-oligosaccharides-1 -14C liberated by glucoamylase...

Malto-oligosaccharides are produced during fermentation via the enzyme-catalyzed breakdown of large polysaccharides and are typically chains of 3-10 glucose monomers. ESI-MS was used to determine the distribution of malto-oligosaccharides in beer.185 Calibration used known concentrations of standard malto-oligosaccharides. Peak areas obtained on injections of diluted beer samples were then compared to the calibration, from which concentrations of malto-oligosaccharides in the beer were determined. 

The relative efficiencies of the malto-oligosaccharides, maltose to maltooc-taose, as acceptors were determined for Leuc. mesenteroides B-512FM dextransu-... 

Fig. 10.9. Electrochromatograms of p-nitrophenyl-a-D-glucopyranosides and malto-oligosaccharides. Column, 200 (270) mm x 100 pm i.d. packed with ODS mobile phase (a), 5 mM NaH2PC>4, pH 6.0-water-acetonitrile 45 45 10 (v/v/v) (b), 5 mM NaH2P04, pH 6.0-water-acetonitrile 42.5 42.5 15 (v/v/v) (c), 5 mil/ NaFfePCU, pH 6.0-water-acetonitrile 40 40 10 (v/v/v). Voltage, 20 kV detection, UV at 254 nm 18°C. Peak identification 1, p-nitrophenyl-a-D-glucopyranoside 2, p-nitrophenyl-a-D-maltoside 3, p-nitrophenyl-a-D-maltotrioside 4, p-nitrophenyl-a-D-maltotctraoside 5, p-nitrophenyl-a-D-maltopentaoside Reproduced with permission from Yang and El Rassi [39].

Malto-oligosaccharides Custom made macroporous polyacrylamide-poly(ethylene glycol) matrix 10 mMTris + 15 mMboric acid (pH 8.2) in acetonitrile-0.1% acetic acid (5 95) 320-500 pm i.d. 250-400 mm packed length 36... 

Elongated (1 —> 4)-linked malto-oligosaccharide chain — (1—>4,1—>-6) branched a-glucan (4.3)... 

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