Oligosaccharides electrophoresis

Zamfir, A., Seidler, D. G., Schonherr, E., Kresse, H. and Peter-Katalinic, J. On-line sheathless capillary electrophoresis/nanoelectrospray ionization-tandem mass spectrometry for the analysis of glycosaminoglycan oligosaccharides. Electrophoresis, 25, 2010, 2004. 

Yang, C. El Rassi, Z. Capillary electrochromatography of derivatized mono- and oligosaccharides. Electrophoresis 1998,19, 2061. 

Rapid Profiling of IgG A -Glycans by Fluorophore-coupled Oligosaccharide Electrophoresis has the Potential of Differentiating Rheumatic Diseases... 

Frears ER, Merry AH and Axford JS. Rapid profiling of IgG N-glycans by fluorophore-coupled oligosaccharide electrophoresis has the potential of differentiating rheumatic diseases. Arth Rheum 1998 41(9) Suppl. S85 No. 317,... 

To allow all culture productiou to be coutrolled, a method for rapid analysis is required. Prior to development of an LC-MS method, the analysis was both complex and time-consuming, involving the purification of a relatively large amount of the antibody using affinity chromatography, enzymatic release, and subsequent derivatizafion of the oligosaccharides and their analysis by using capillary electrophoresis. 

Figure 21 Separation of ANTS-labeled oligosaccharides. (From Guttman, A., Cooke, N., and Star, C. M., Electrophoresis, 15, 1518, 1994. With permission.)...

Suzuki, S., Kakehi, K., and Honda, S., Two-dimensional mapping of N-gly-cosidically linked asialo-oligosaccharides from glycoproteins as reductively pyridylaminated derivatives using dual separation modes of high-performance capillary electrophoresis, Anal. Biochem., 205, 227, 1992. 

Guttman, A. and Pritchett, T., Capillary gel electrophoresis separation of high-mannose type oligosaccharides derivatized by l-aminopyrene-3,6,8-trisul-fonic acid, Electrophoresis, 16, 1906, 1995. 

Camilleri, P., Harland, G. B., and Okafo, G., High resolution and rapid analysis of branched oligosaccharides by capillary electrophoresis, Anal. Biochem., 230, 115,1995. 

Pathogen-suppressing oligosaccharides in human milk were quantitated using capillary Electrophoresis by MEKC with detection by absorption at 205 nm.90 A 24 kDa glycoprotein associated with muscle-wasting cachexia... 

Shen, Z., Warren, C.D., and Newburg, D.S., High-performance capillary electrophoresis of sialylated oligosaccharides of human milk, Anal. Biochem., 279, 37, 2000. 

After the release of the oligosaccharides, they must be purified by a variety of methods before structural analyses can be undertaken. Ion-ex-change chromatography, gel-filtration chromatography, or some type of electrophoresis is usually used for the purification. At this point, structural analyses may begin. 

Kakehi, K., Kinoshita, M., and Nakano, M. 2002. Analysis of glycoproteins and the oligosaccharides thereof by high performance capillary electrophoresis - significance in regulatory studies on biopharmaceutical products. Biomedical Chromatography 16(2), 103-115. 

B. Lagane, M. Treilhou and F. Couderc, Capillary electrophoresis theory, teaching approach and separation of oligosaccharides using indirect UV detection. Biochem. Mol. Biol. Educ. 28 (2000) 251-255. 

Zamfir A, Seidler DC, Kresse H, Peter-Katalinic J. 2002. Structural characterization of chondroitin/dermatin sulfate oligosaccharides from bovine aorta by capillary electrophoresis and electrospray ionization quadrupole time-of-flight tandem mass spectrometry. Rapid Commun Mass Spectrom 16 2015. 

Taverna, M., Baillet, A., Biou, D., Schluter, M., Werner, R., and Terrier, D. (1992). Analysis of carbohydrate-mediated heterogeneity and characterization of N-linked oligosaccharides of glycoproteins by high-performance capillary electrophoresis. Electrophoresis 13, 359—366. 

Suzuki, H., Muller, O., Guttman, A., and Karger, B. L. (1997). Analysis of 1-aminopyrene 3,6,8-trisulfonate-derivatized oligosaccharides by capillary electrophoresis with matrix- assisted laser desorptlon/ionization tlme-of-fhght mass spectrometry. Anal. Chem. 69, 4554—4559. 

A D Hulst, N Verbecke. Chiral separation by capillary electrophoresis with oligosaccharides. J Chromatogr 608 275-287, 1992. 

H Soini, M Stefansson, M-L Riekkola, MV Novotny. Malto-oligosaccharides as chiral selectors for the separation of pharmaceuticals by capillary electrophoresis. Anal Chem 66 3477-3484, 1994. 

A D Hulst, N Verbeke. Chiral analysis of basic drugs by oligosaccharide-mediated capillary electrophoresis. J Chromatogr A 735 283—293, 1996. 

Before discussing the various derivatives that have been used, it should be remembered that oligosaccharides are often obtained by chromatography on paper or on cellulose columns, and this may cause them to become contaminated by xylan this may be eliminated by extraction of the crude fractions with hot ethanol.474 For similar reasons, glucose that is really extraneous may be detected in fractions separated by electrophoresis on cellulose acetate.475 Gas-liquid chromatography was used to show the presence in pituitary glyco-... 

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