Oligonucleotide Delivery

PESDA/Oligonucleotide attached by adsorption Oligonucleotide delivery [82]... 

B. Tavitian, S. Marzabal, V. Boutet, B. Kuhnast, S. Terrazzino, M. Moynier, F. Dolle, J.R. Deverre, A.R. Thierry, Characterization of a synthetic anionic vector for oligonucleotide delivery using in vivo whole body dynamic imaging, Pharm. Res. 19 (2002) 367-376. 

Zimmer, A. (1999). Antisense oligonucleotide delivery with polyhexylcyanoacrylate nanoparticles as carriers. Methods, 18(3), 286-295, 322. 

Poly(L-lysine) (PLL) Linear, tends to form secondary structures, primary amino groups Plasmid and oligonucleotide delivery, in vitro and in vivo (WuandWu, 1987, Wagner eto/., 1991)... 

Pluronic-g- polyethyleneimine (Pluronic-g-PEI) Graft-copolymer, branched PEI segment grafted with nonionic triblock copolymer In vitro and in vivo transfections and oligonucleotide delivery (Nguyen etal., 2000)... 

Kabanov, A.V., Vinogradov, S.V., Suzdaltseva, Y.G. and Alakhov, V.Y. (1995) Water-soluble block polycations as carriers for oligonucleotide delivery. Bioconjug. Chem., 6, 639-643. 

For oligonucleotide delivery to eukaryotic cells compound GS2888 (Figure 15.17) was synthesized by Lewis et al. (1996). This compound can be used both in the presence and absence of serum with high reproducibility and minimal toxicity. 

Oligonucleotide delivery with [S-CD for targeted cancer therapeutics has been explored extensively by the group of Mark Davis, and the remainder of this section is focused on the groundbreaking in vivo results attained by this laboratory. Previous work in this lab has extensively exploited the hydrophobic interior cavity of [5-CD for inclusion of hydrophobic molecules conjugated to targeting groups... 

Matsumoto T, Numata M, Anada T et al (2004) Chemically modified polysaccharide schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake efficiency. Biochem Biophys Acta 1670 91-104... 

Photochemical Internalization A New Tool for Gene and Oligonucleotide Delivery... 

Vinogradov SV, Batrakova EV, Kabanov AV (2004) Nanogels for oligonucleotide delivery to the brain. Bioconjug Chem 15 50-60. 

Krotz F, de Wit C, Sohn HY, Zahler S, Gloe T, Pohl U, Plank C (2003) Magnetofection -a highly eflficient tool for antisense oligonucleotide delivery in vitro and in vivo. Mol Ther 7 700-710... 

The cationic peptide family includes both synthetic and naturally derived peptides, all of which have been successfully employed for both in vitro and in vivo delivery of a variety of macromolecules (Table 13.1). The prototypic example of a naturally derived cationic peptide is the Tat PTD, described above. Other examples include the pVEC peptide, which has been successfully used for both protein and oligonucleotide delivery,40 as well as a series of small, RNA-binding peptides discovered by Futaki and colleagues.20 The Penetratin vector is also included in this group. The inclusion of Tat and Penetratin in the cationic peptide group arises from the intrinsic definition of these peptides. In general, the cationic delivery peptides... 

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