Saccharomyces cerevisiae of

Dining batch fermentation of Saccharomyces cerevisiae, other influential parameters can adversely influence the specific rate of growth, and inhibition can be caused either by... 

Seed culture of Saccharomyces cerevisiae (ATCC 24860) is grown in a rich medium comprising of 1 g glucose, 0.1 g peptone and yeast extract, 0.33 g KH2P04 and 0.03 g Na2HP04 in 100 ml distilled water. The media will be autoclaved at 126 °C and 15 psig for 20 min. The stock culture from ATCC media is transferred to a prepared seed culture. The pH of... 

Fig. 10.2. Standard curve for cell density define based on growth of Saccharomyces cerevisiae.

Preparation by fermentation of Saccharomyces cerevisiae (baker yeast) with addition of L- or DL-methionine, lyse of cells with ethyl acetate and purification by ion-exchange chromatography. 

Fig. 2.2 Landmark events in the cell cycle of Saccharomyces cerevisiae. Gl, S, G2 and M are the classical phases of the eukaryotic cell cycle.

Andreasen A. A. Stier J.B. (1953) Anaerobic nutrition of Saccharomyces cerevisiae. I. Ergosterol requirement for growth in a defined medium. / Cell Comp Physiol, 41, 23-36. 

Dickinson J.R., Dawes I.W., Boyd A.S.F. Baxter R.L. (1983) C NMR studies of acetate metabolism during spomlation of Saccharomyces cerevisiae. Proc Nat Acad Sci USA, 80, 5847-5851. 

Van Rinsum J., Klis EM. van den Ende H. (1991) Cell wall glucomannoproteins of Saccharomyces cerevisiae mnn9. Yeast, 7, 717-726. 

Intrinsic (natural, innate) resistance. In one form of intrinsic resistance, the fungal cell wall (see Chapter 2) is considered to present a barrier to exclude or, more likely, to reduce the penetration by biocide molecules. The evidence to date is sketchy but the available information tentatively links cell wall glucan, wall thickness and consequent relative porosity to the sensitivity of Saccharomyces cerevisiae to chlorhexidine. 

Drdak, M. et al., Red beet pigment composition effects of fermentation by different strains of Saccharomyces cerevisiae, J. Food Sci., 57, 935, 1992. 

The notion that the segment containing C3 and T3 is important for conformational adaptability of the protein is supported by mutations in yeast. Mutations of the genes of the H-ATPase of Saccharomyces cerevisiae resulted in a thermo-sensitive... 

Yeasts contain a large number of different active and passive sugar-transport systems. The first of these to be cloned was the glucose-repressible, high-affinity passive glucose transporter of Saccharomyces cerevisiae, which is encoded by the SNF3 gene... 

From a genetical point of view, Saccharomyces cerevisiae is an ideal organism which may be considered the Escherichia coli of eukaryotic cells [4,5]. This is true in particular for the study of metabolic regulation and for that of membrane transport [6]. Finally, the astonishing resemblance between many yeast proteins and certain mammalian-cell proteins has seriously broadened the scope of interest. Although a few reports have appeared on amino acid transport in some other yeasts, most investigations in this field have used strains of Saccharomyces cerevisiae. 

An example of transporter identification in a complex case the three GABA transport systems of Saccharomyces cerevisiae... 

Despite the limited information available, rather clear predictions can be made about the probable structure, location, and energy coupling of the amino acid transporters of Saccharomyces cerevisiae, by comparing them with better known systems in both prokaryotes and eukaryotes. 

The proline transport protein prnB of Aspergillus nidulans [47] is very similar to the above-mentioned family of Saccharomyces cerevisiae amino acid transporters (about 42% identity with the PUT4 gene product and 30% identity with the CANl and the HIP I gene products). So is the AroP general aromatic amino acid transporter protein of Escherichia coli K-12, which has about 30% identity with the HIPI gene product [48]. Both hydrophilic ends are very different from one transporter to another (see Fig. 2). 

The aryl hydroxylase of Saccharomyces cerevisiae that transforms benzo[a]pyrene to the 3- and 9-hydroxy compounds, and the 7,8-dihydrodiol (King et al. 1984). 

Dinardo S, Voelkel K, Stemglanz R 1984 DNA topoisomerase II mutant of Saccharomyces cerevisiae. topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication. Proc Natl Acad Sci USA 81 2616-2620... 

Figure 13.5 Mass defect plot of a 9.4 T MALDI-FTMS spectrum of Saccharomyces cerevisiae.

Kaluzna, I.A., Matsuda, T., Sewell, A.K. and Stewart, J.D. (2004) Systematic investigation of Saccharomyces cerevisiae enzymes catalyzing carbonyl reductions. Journal of the American Chemical Society, 126 (40), 12827-12832. 

Kuranda, M.J. and Robbins, P.W. (1991) Chitinase is required for cell separation during growth of Saccharomyces cerevisiae. Journal of Biological Chemistry 266, 19758-19767. 

Jelinsky SA et al. Global response of Saccharomyces cerevisiae to an alkylating agent. Proc Natl Acad Sci USA 1999 96 1486-1491. 

Pardo M et al. A proteomic approach for the study of Saccharomyces cerevisiae cell wall biogenesis. Electrophoresis 2000 21 3396-3410. 

Kuhn, K. M., DeRisi, J. L., Brown, P. O., and Samow, P. (2001). Global and specific translational regulation in the genomic response of Saccharomyces cerevisiae to a rapid transfer from a fermentable to a nonfermentable carbon source. Mol. Cell Biol. 21, 916-927. 

Unless otherwise stated, all experiments were done in liquid media and with free biomass Obsolete name for Cryptococcus curvatus bSynomyms of Saccharomyces cerevisiae... 

TSAY, Y.H., ROBINSON, G.W., Cloning and characterization of ERG8, an essential gene of Saccharomyces cerevisiae that encodes phosphomevalonate kinase, Mol. Cell. Biol., 1991,11,620-631. 

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