Spatial Screening of Lectin Ligands

To accelerate the process of finding the required presentation of carbohydrates when no structural information on the receptor is available, we developed a screening procedure for multivalent lectin ligands comprising four steps [15-17]  

split-mix synthesis (Box 11) of a library of scaffold molecules containing side-chain amino groups in varying amounts and spatial orientation  

attachment of several copies of a carbohydrate ligand to the amino groups  

on-bead screening of the library for lectin-binding properties and 

identification of potent ligands by single-bead analysis. 

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Fig. 2.7.6. Structure of multivalent neoglycopeptides used for spatial screening of lectin ligands [15, 17].

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