Labeling site-specific

To clarify the purity, labeling site, specific radioactivity, stability, the guidance on repeated dose tissue distribution studies is to be referred in the Notification No. 442 of the PAB in July 1996. 

Site-specific deuterium labeling of steroids can also be accomplished by biosynthetic procedures, using deuterated precursors, but these methods are beyond the scope of this chapter. For reviews of this subject see, for example, ref. 45, 54 and 55. 

Pintacuda, G., Moshref, A., Leonchiks, A., Sharipo, A., Otting, G. Site-specific labeling with a metal chelator for protein-structure refinement. /. Biomol. NMR 2004, 29, 351-361. 

G. Site-specific labeling of proteins with a rigid lanthanide-binding tag. ChemEioChem 2006, 7,1599-1504. 

To get site-specific information about this unfolding process, a similar series of peptides, Ac-(AAAAK)3AAAA-YNH2, was sequentially labeled with 13C on the C=0 of four Ala residues, first on those at the N terminus, and then sequentially for the three other tetra-Ala positions (Decatur and Antonie, 1999). IR results showed a band shifted down 40 cm-1 from the amide F, which formed a clearly resolved band... 

Goodison, S., Kawai, K., Hihara, J., Jiang, P., Yang, M., Urquidi, V., Hoffman, R. M., Tarin, D. (2003). Prolonged dormancy and site-specific growth potential of cancer cells spontaneously disseminated from nonmetastatic breast tumors as revealed by labeling with green fluorescent protein. Clin. Cancer Res. 9(10), 3808-3814. 

Chattopadhaya S, Srinivasan R, Yeo DSY et al (2009) Site-specific covalent labeling of proteins inside live cells using small molecule probes. Bioorg Med Chem 17 981-989... 

Bark, S. J. and Hahn, K. M. (2000). Fluorescent indicators of peptide cleavage in the trafficking compartments of living cells Peptides site-specifically labeled with two dyes. Methods 20, 429-435. 

Reported applications of SADP include the crosslinking of con A to receptors oVn human erythrocyte membranes (Vanin and Ji, 1981), site-specific labeling of lectins using modified... 

Baenzigen J.U., and Fiete, D. (1982) Photoactivatable glycopeptide reagents for site-specific labeling of lectins./. Biol. Chem. 257, 4421M425. 

Thevenin, B., Shahrokh, Z., Williard, R., Fujimoto, E., Ikemoto, N., and Shohet, S. (1991) A novel reagent for functionally-directed site-specific fluorescent labeling of proteins, Abstract. Biophys. J. 59, Tu-Pos 476, p. 358a. 

The use of deuterated organosilicon hydrides in conjunction with proton acids permits the synthesis of site-specific deuterium-labeled compounds.59 126 221 Under such conditions, the deuterium atom in the final product is located at the charge center of the ultimate carbocation intermediate (Eq. 62). With the proper choice of a deuterated acid and organosilicon hydride, it may be possible to use ionic hydrogenation in a versatile manner to give products with a single deuterium at either carbon of the original double bond, or with deuterium atoms at both carbon centers.127... 

Jones DH, Cellitti SE, Hao X, Zhang Q, Jahnz M, Summerer D, Schultz PG, Uno T, Geierstanger BH (2010) Site-specific labeling of proteins with NMR-active unnatural amino acids. J Biomol NMR 46 89-100... 

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