Nucleic acid recognition

Nucleic acid recognition by metal complexes of bleomycin 99CRV2797. 

Preorganization of DNA and improving nucleic acid recognition by synthetic oligonucleotides 97CRV1473. 

Klug A, Rhodes D (1987) Zinc finger a novel protein motif for nucleic acid recognition. Trends Biochem Sci 12 464... 

Combinatorial chemistry has been used with great success to create libraries in the development of inhibitors in the field of peptide and nucleic acid recognition.5,6 The basic strategy of a library approach is to synthesize large sets of molecules at a time, even as complex mixtures, and then determine whether any of the compounds is inhibitory. The active compound must be subsequently identified. This strategy stands in contrast to the extremely laborious and expensive process of traditional medicinal chemistry, where individual molecules are carefully synthesized and evaluated. The... 

Auld, D.S. (2001) Zinc coordination sphere in biochemical zinc sites, BioMetals, 14, 271-313. Klug, A. and Rhodes, D. (1988) Zinc fingers a novel protein motif for nucleic acid recognition, TIBS, 12, 464-469. 

The use of nucleic acids recognition layers is a new and exciting area in analytical chemistry which requires extensive research. 

Carbohydrates and glycosides recently emerged as a novel class of nucleic acid binding compounds. A detailed study of the factors affecting the site-selectivity of some recently discovered antitumor antibiotics has shed new light on the role that oligosaccharides may play in nucleic acid recognition. 

The course of a number of other photoreactions could be altered or oriented by selective binding. In particular, substances effecting single- or double-strand nucleic acid recognition and fitted with a photoreactive group may perform sequence specific photocleavage [4.56, 5.29, 5.30]. 

The use of nucleic acids recognition layers represents a new and exciting area in analytical chemistry which requires an extensive study. Besides classical methodologies to detect DNA, novel approaches have been designed, such as the DNA chips [10-12] and lab-on-a-chips based on microfluidic techniques [13]. However, these technologies are still out of the scope of food industry, since it requires simple, cheap and user-friendly analytical devices. 

Computational biochemistry and computer-assisted molecular modeling have rapidly become a vital component of biochemical research. Mechanisms of ligand-receptor and enzyme-substrate interactions, protein folding, protein-protein and protein-nucleic acid recognition, and de novo protein engineering are but a few examples of problems that may be addressed and facilitated by this technology. 

Specific Protein-Nucleic Acid Recognition Involves Hydrogen Bonding... 

Three levels of protein-nucleic acid recognition have been observed. Nature provides three examples of protein-nucleic acid interactions which we shall consider. The nucleic acid component can be (1) a single nucleotide, e.g., a coenzyme or a substrate, (2) a single-stranded DNA or RNA as in ribonucleases A and T, or (3) a double-stranded DNA or RNA as in the highly specific complexes with repressors in the tRNA-synthetase complex, or in the unspecific nuclease DNase I. 

Keywords DNA G-quadruplex Nucleic acid recognition Resin-bound DCC RNA Transition metal complex... 

Inorganic Chemistry in Biology Oxidative DNA Damage, Chemistry of Physico-Chemical Properties of Nucleic Acids Nucleic Acid Recognition by Peptides and Drugs DNA Damage and Carcinogenesis... 

Nucleic Acids, Chemistry of Nucleic Acids, Design and Engineering of Nucleic Acid Recognition by Peptides and Drugs Synthetic Chemistry Building Molecules to Modulate Biological Systems... 

Singh SK, Nielsen P, Koshkin A, Wengel J. LNA (locked nucleic acids) Synthesis and high-affinity nucleic acid recognition. Chem. Commun. 1998 455-456. 

RR duplexes and the derived triplexes. This could be a consequence of stable duplex structures which need more energy to become distorted to accommodate the third strand. The stability order of various RNA-DNA hybrid triplexes indicates that duplexes with DNA in the purine strand readily bind to either RNA or DNA to form triplexes. In contrast, a purine RNA duplex binds only RNA on a third strand. It was recently shown that RNA cannot be a part of a triplex with the R RY motif [92c]. These studies point to the fundamental role of sugars in controlling nucleic acid recognition through conformational switches. Since RNA-DNA hybrids are cellular targets for a number of... 

---