Photoreactive groups

Anthraquinone groups are highly photoreactive by exposure to long UV light ranging from 340 to 360 nm. Unlike photoreactive groups that form intermediate nitrene or carbine precursors... 

Psoralen, or derivatives of 9-methoxy-7H-furo[3,2-g]chromen-7-one tricyclic ring structures, are used as photoreactive groups in crosslinkers, biotinylation compounds, and nucleic acid probes. Psoralens have been used for many years as photochemotherapy agents for treatment of psoriasis and vitiligo (Smith and Barker, 2006). Psoralens react when exposed to UV light... 

In use, SAND is first reacted with an amine-containing protein or other molecule—being careful to protect the photoreactive group from inadvertent degradation by exposure to excessive room light or sun. The modified intermediate then is allowed to interact with a target molecule. Finally, the photolyzing process is done to effect a nonselective crosslink between the... 

Psoralen-PEOj-Biotin has been used to label double-stranded DNA for detection using (strept)avidin reagents (Henriksen et al., 1991 Wygrecka et al., 2007). The psoralen photoreactive group provides better insertion yields than typical phenyl azide-based systems, such as the standard photobiotin probe discussed previously in this section. 

Sulfo-SAND, SANPAH, and Sulfo-SANPAH all contain a nitrated phenyl azide photoreactive group. The presence of the nitro group shifts the optimal wavelength for photoactivation... 

The following references provide further information on the use of Sulfo-SFAD and perfluorophenyl azide photoreactive groups Pandurangi et al. (1995a, b, 1996, 1997a, b, 1998) Yan etal. (1994). 

Introduction of a photoreactive group to biologically active molecules and setting all the parameters for the experiment. 

There are several photoreactive groups and structural units ( photophores ) used in PAL [3,4,6,8]. The main criteria for the selection are ... 

The photoreactive groups should have sufficient stability under ambient light. 

Photoreactive Groups for Identifying and Regulating Protein Activity... 

Genetic Incorporation of Unnatural Amino Acids into Proteins 609 5.18.3.3 Photoreactive Groups for Identifying and Regulating Protein Activity... 

Surface-introduced photoreactive group, aryl diazirine, can generate carbenes by 365 nm UV exposure. However, gold-sulfiir bonds, which support photolinker on gold, can be easily broken by UV light below 300 nm. Therefore, UV transmission filter is essential in UV irradiation. 

The course of a number of other photoreactions could be altered or oriented by selective binding. In particular, substances effecting single- or double-strand nucleic acid recognition and fitted with a photoreactive group may perform sequence specific photocleavage [4.56, 5.29, 5.30]. 

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