Conformational switching

Patch, ).A., Wu, C.W., Sanborn, T.J., Huang, K., Zuckermann, R.N., Barron, A. E., and Radhakrishnan, I. A peptoid oligomer with a unique, cyclic folded structure and a solvent-depend conformational switch. (Manuscript in preparation). 

Just like in coiled-coils, p-sheet secondary structure (Fig. 2) is ubiquitous in natural examples and in proteins and biomaterials. Alzheimer s disease is characterized by fibrillar amyloid plaques in the cerebral parenchyma. The insoluble amyloid fibrils are predominantly formed upon conformational switching of the 42 amino acid... 

Yang JJ, Pitkeathly M, Radford SE (1994) Far-uv circular-dichroism reveals a conformational switch in a peptide fragment Irom the beta-sheet of hen lysozyme. Biochemistry 33 7345-7353... 

Figure 14-11. Snapshots from the conformational switch path explored in the vicinity of the active conformation (unfolded docked conformation) and starting from the inactive (undocked) conformation found in crystal. Stems A and B were aligned (best fitted) and are shown in yellow, different instances of stem C are shown in stick representation with different colors. A schematic of the non-canonical binding scheme of the ligation site is shown in the right panel and the general mechanism of ligation in the left panel...

Petitjean A, Lehn JM (2007) Conformational switching of the pyridine-pyrimidine-pyridine scaffold for ion-controlled FRET. Inorganica Chim Acta 360 849-856... 

Rahimi M, Troisi A (2009) Probing local electric field and conformational switching in single-molecule break junctions. Phys Rev B 79 113413-113414... 

Prioleau, C., Visiers, I., Ebersole, B. J., Weinstein, H., and Sealfon, S. (2002) Conserved helix7 tyrosine acts as a multistate conformational switch in the 5-HT2C receptor Identification of a novel locked-on phenotype and double revertant mutations. J. Biol. Chem. 277, 36577-36584. 

Krishna, A. G., Menon, S. T., Terry, T. J., and Sakmar, T. P. (2002) Evidence that helix 8 of rhodopsin acts as a membrane-dependent conformational switch. Biochemistry 41, 8298-8309. 

The Fe-protein has the protein fold and nucleotide-binding domain of the G-protein family of nucleotide-dependent switch proteins, which are able to change their conformation dependent on whether a nucleoside diphosphate (such as GDP or ADP) is bound instead of the corresponding triphosphate (GTP or ATP). However, nucleotide analogues, which induce the conformational switch of the Fe-protein, do not allow substrate reduction by the MoFe-protein, nor does reduction of the MoFe-protein by other electron-transfer reagents (whether small proteins or redox dyes) drive substrate reduction. Only the Fe-protein can reduce the MoFe-protein to a level that allows it to reduce substrates such as... 

Scheme 14 Yamada s chiral conformation-switch catalyst applied to the KR of aryl alkyl sec-alcohols [136, 144]...

Gabashvili IS, Agrawal RK, Grassucci R, Squires CL, Dahlberg AE, Frank J (1999) Major rearrangements in the 70S ribosomal 3D structure caused by a conformational switch in 16S ribosomal RNA. EMBO J 18 6501-6507... 

Lodmell JS, Dahlberg AE (1997) A conformational switch in Escherichia coli 16S ribosomal RNA during... 

Du Q, Macara IG (2004) Mammalian Pins is a conformational switch that links NuMA to heterotrimeric G proteins. Cell 119 503-516... 

A similar peptidic conformational switch was developed by Mutter and Hersperger [9]. In that work, they showed that the 15-residue peptide 7 could have its conformation modulated by the polarity of the solvent (Fig. 6). Indeed, peptide 7 was shown to adopt an a-helical structure in trifluoroethanol and... 

Kisselev, O., Pronin, A., Ermolaeva, M., and Gautam, N. (1995). Receptor-G protein coupling is established by a potential conformational switch in the / y complex. Proc. Natl. Acad. Sci. USA 92, 9102-9106. 

Coiled-coil motifs have been known to play roles in conformational switching in natural proteins for some time (Oas and Endow, 1994). The key examples are influenza hemagglutinin (Bullough et al., 1994 Carr and Kim, 1993 Carr et al., 1997), and the heat shock transcription factor (Rabindran et al., 1993). Furthermore, an engineered form of GCN4-pl, with Asn-16 replaced by Ala, switches from dimer to trimer upon addition of... 

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