Nucleic acid reduced

DNAse and RNAse 1 pg/ml degradation of nucleic acids, reduce viscosity of sample solution... 

Nucleic acid contents of SCP products, which range up to 16% in bacteria and 6—11% in yeasts, must be reduced by processing so that intakes are less than 2 g/d to prevent kidney stone formation or gout. Adverse skin and gastrointestinal reactions have also been encountered as a result of human consumption of some SCP products (87). 

On homogenization, the lysate may drastically increase in viscosity due to DNA release. This can be ameliorated to some extent using multiple passes to reduce the viscosity. Alternatively, precipitants or nucleic acid digesting enzymes can be used to remove these viscosity-enhancing contaminants. 

The removal and reduction of the nucleic acid content of various SCPs is achieved by chemical treatment with sodium hydroxide solution or high salt solution (10%). As a result, crystals of sodium urate form and are removed from the SCP solution.16,17 The quality of SCP can be upgraded by the destruction of cell walls. That may enhance the digestibility of SCP. With chemical treatment the nucleic acid content of SCP is reduced. 

D. J., Douglas C. L, Boules M., Stewart J.A., Zhao L., Lacy B., Cusack B., Fauq A., Richelson E. Peptide nucleic acids targeted to the neurotensin receptor and administered i.p. cross the blood-brain barrier and specifically reduce gene expression. Proc. Natl Acad. Sci. USA 1999 96 7053-7058. 

Decreased cerebral blood flow, resulting from acute arterial occlusion, reduces oxygen and glucose delivery to brain tissue with subsequent lactic acid production, blood-brain barrier breakdown, inflammation, sodium and calcium pump dysfunction, glutamate release, intracellular calcium influx, free-radical generation, and finally membrane and nucleic acid breakdown and cell death. The degree of cerebral blood flow reduction following arterial occlusion is not uniform. Tissue at the... 

Kasai, H. and Nishimura, S. (1984). Hydroxylation of deox-yguanosine at the C-8 position by ascorbic acid and other reducing agents. Nucleic Acids Res. 12, 2137-2145. 

The most important product of the hexose monophosphate pathway is reduced nicotinamide-adenine dinucleotide phosphate (NADPH). Another important function of this pathway is to provide ribose for nucleic acid synthesis. In the red blood cell, NADPH is a major reducing agent and serves as a cofactor in the reduction of oxidized glutathione, thereby protecting the cell against oxidative attack. In the syndromes associated with dysfunction of the hexose monophosphate pathway and glutathione metabolism and synthesis, oxidative denaturation of hemoglobin is the major contributor to the hemolytic process. 

In this respect, an interesting approach to reduce degradation and possible toxicity problems related to nucleic acid use in vivo is offered by their encapsulation in or association to microcarrier systems, such as neutral or cationic liposome and polymeric microparticles [41 14],... 

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