Nicotiana sylvestris

Dix, P.J. Pearce, R.S. (1981). Proline accumulation in NaCI-resistant and sensitive cell lines of Nicotiana sylvestris. Zeitschrift fur Pflanzenphysiologie, 102, 243-8. 

Loughrin, J. H Hamilton-Kemp, T.D, Andersen, R. A. and Hildebrand, D.F. (1991). Circadian rhythm of volatile emission from flowers of Nicotiana sylvestris and N. suaveolens. Physiologia Plantarum 83 492-496. 

BALDWIN, I.T., SCHMELZ, E.A., OHNMEISS, T.E., Wound induced changes in root and shoot jasmonic acid pools correlate with induced nicotine synthesis in Nicotiana sylvestris. J. Chem. Extol., 1994,20,2139-2158. 

MCCLOUD, E.S., BALDWIN, I.T., Herbivory and caterpillar regurgitants amplify the wound-induced increases in jasmonic acid but not nicotine in Nicotiana sylvestris. Planta, 1997,203,430-435. 

VOELCKEL, C., KRUEGEL, T., GASE, K HEIDRICH, N VAN DAM, N.M., WINZ, R., BALDWIN, I.T., Anti-sense expression of putrescine N-methyltransferase confirms defensive role of nicotine in Nicotiana sylvestris against Manduca sexta Chemoecology, 2001,11,121-126. 

Sedimentation of amyloplasts within the cell has been correlated with the capacity of the plant to perceive gravity. The buoyant mass of amyloplasts present in specialized cells in the center of the root cap and in the stem (depending on the plant species, in the endodermis, the bundle sheath, or in the parenchyma to the inside of the vascular bundle) would allow the amyloplasts to sediment inside the cell, where the cytosol would have a relatively low viscosity. This sedimentation would translate into a signal of an unknown nature, maybe through pressure onto a sensitive part of the cell or acting as a mechano transducer, etc. Whatever the nature of the signal, it eventually results in the asymmetry of the organ and its curvature. The isolation of starchless mutants of Arabidopsis thaliana and Nicotiana sylvestris has made... 

Genschik, R, Criqui, M.-C., Parmentier, Y, Marbach, J., Durr, A., Fleck, J. and Jamet, E. (1992) Isolation and characterization of a cDNA encoding a 3-hydroxy-3-methylglutaryl coenzyme A reductase from Nicotiana sylvestris. Plant Mol. Biol., 20, 337-41. 

Ghashgjiaie J., Duranceau M., Badeck F. W., Comic G., Adeline M. T., and Deleens E. (2001) 8 C of CO2 Respired in the dark in relation to 8 C of leaf metabolites comparison between Nicotiana sylvestris and Helianthus annuus under drougjit. Plant Cell Environ. 24(5), 505-515. 

Pollen germination and pollen tube growth have been used as end points. Pollen grains from a number of plants have been used, and an assay for growth of pollen from Nicotiana sylvestris has been developed (Kristen and Kappler 1995). 

The overall goal of the TGI is to sequence and annotate more than 90% of the open reading frames in the genome of cultivated tobacco, Nicotiana tabacum. Nicotiana tabacum is an amphiploid species (2n = 48) likely resulting from an interspecific cross between Nicotiana sylvestris (2n = 24) and Nicotiana tomentosiformis (2n = 24), and at approximately... 

Carboxylase, ribulose diphosphate (Nicotiana sylvestris ctone NySS4 small subunit precursor reduced) 2236a, 3973, 4249... 

Genschik, P, A. Durr, and J. Eleck Differential expression of several E2-type ubiquitin carrier protein genes at different developmental stages in Arabidopsis thaliana and Nicotiana sylvestris Molecular Gen. Genetics 244 (1994) 548-556. 

Wada, E. Chemical constituents of tobacco. HI. Kaempferol-3-rhamnoglucoside from the flowers of Nicotiana sylvestris. 

Deoxyribonucleic acid (Nicotiana sylvestris clone NySS4 ribulose diphosphate carboxylase small subunit gene) XXII-2 ... 

Neuhaus, J.M., Flores, S., Keefe, D., Ahi-Goy, P. and Meins, F., Jr. (1992). The function of vacuolar beta-1,3-glucanase investigated by antisense transformation. Susceptibility of transgenic Nicotiana sylvestris plants to Cer-cospora nicotianae infection. Plant Mol. Biol. 19, 803-813. 

The wild-growing tobacco variety Nicotiana sylvestris reacts to mechanical damage, for example by herbivores like the caterpillar of the American tobacco hornworm Manduca sexto, by increasing its nicotine production up to four-fold (Fig. 5.206). The tobacco farmer pursues the same objective bypinching out the shoots of young plants. Their response is mediated by jasmonic add. Unharmed tobacco plants show higher nicotine levels as well, if the phytohormone is supplied e.g. with irrigation water to the roots. [537,538]... 

Nicotiana sylvestris (a) and the caterpillar of the American tobacco hornworm (Manduca sexta) (b). 

An average 60-day-old tobacco plant contains about 250 mg of nicotine. Based on the net carbon dioxide fixation and the rate of turnover of nicotine in the plant, maintenance of this amount of nicotine may represent as much as 10% of the plant s total metabolism (Robinson, 1974). The half-life of nicotine in tobacco plants is about 22 h (Waller and No-wacki, 1978). The alkaloid content of plants of Nicotiana sylvestris undergoes a fourfold increase following damage to the leaves (Hartmann, 1991). The production of nicotine and related pyridine alkaloids such as anabasine (45), anatabine (46), and nornicotine (47) in plant tissue, cell, and hairy root cultures has been reviewed (Verpoorte et al., 1991). 

From the available data it can be tentatively concluded that the level and/or metabolism of endogenous cytokinins changes markedly, often transiently, at the time of floral transition in many plant species [1]. The trend of changes was, however, opposite in species with different photoperiodic requirements an increase in levels was recorded, for example, in leaves of the LDP Hyoscyamus and Nicotiana sylvestris and the SDP Begonia, whereas a decrease was observed in the SDP Xanthium and Chenopodium [see 5, 11, 16]. 

PMT expression occurs in the root pericycle of A. belladonna L. and H. muticus L. [2, 36, 37] and in endodermis, outer cortex, and xylem in Nicotiana sylvestris L. [36, 37]. The localization of PMT to the pericycle would facilitate the access to arginine 12 or ornithine 8 [2,6]. In the same way, A -methylputrescine 13 or some biosynthetic intermediate could then be transported to the endodermis for further elaboration into tropine 27 [6]. 

Siva Raju K, Krishnamurthy GVG (1996) Biochemical changes in tobacco plants infested with root-knot nematodt Meloidogyne javanica. Tobacco Res 22 116-119 Smith CR (1937) Occurrence of /-nomicotine in Nicotiana sylvestris. J Econ Entomol 20 724-727... 

Baldwin IT, Hamilton W (2000) Jasmonate-induced responses of Nicotiana sylvestris results in fitness costs due to impaired competitive ability for nitrogen. J Chem Ecol 26 915-952. 

Shoji T, Yamada Y, Hashimoto T (2000) Jasmonate induction of putrescine N-methyltransferase genes in the root of Nicotiana sylvestris. Plant Cell Physiol 41 831-839... 

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