Neutrophil adhesins

The peroxidase-negative granules produced later in neutrophil development are termed secondary or specific granules (because they stain only with specific dyes). They are twice as abundant in the cytoplasm of the mature neutrophil as the azurophilic granules. Specific granules contain much of the lysozyme, lactoferrin, vitamin-B 12-binding protein, adhesin receptors, fMet-Leu-Phe receptors, and cytochrome b.245. 

Petersson, C., Porsberg, M., Aspholm, M., Olfat, F. O., Forslund, T., Boren, T., and Magnusson, K. E. (2006). Helicobacter pylori SabA adhesin evokes a strong inflammatory response in human neutrophils which is down-regulated by the neutrophil-activating protein. Med. Microbiol. Immunol. 195,195-206. 

Unemo, M., Aspholm-Hurtig, M., liver, D., Bergstrom, J., Boren, T., Danielsson, D., and Teneberg, S. (2005). The sialic acid binding SabA adhesin of Helicobacter pylori is essential for nonopsonic activation of human neutrophils. J. Biol. Chem. 280, 15390-15397. 

Abstract Persistent colonization of the human stomach by Helicobacter pylori is a risk factor for the development of peptic ulcer disease and gastric cancer. Adhesion of microbes to the target tissue is an important determinant for successful initiation, establishment and maintenance of infection, and a variety of different candidate carbohydrate receptors for H. pylori have been identified. Here the different the binding specifities, and their potential role in adhesion to human gastric epithelium are described. Finally, recent findings on the roles of sialic acid binding SabA adhesin in interactions with human neutrophils and erythrocytes are discussed. 

It should, however, be noted that despite this multitude of potential carbohydrate receptors, only two H. pylori adhesins have been identified to date, i.e., the Leb-binding adhesin BabA [16], and the sialic acid-binding adhesin SabA [8], An additional H. pylori carbohydrate binding protein is the soluble neutrophil-activating protein... 

Fig. 3 Luminol-enhanced chemiluminescence of human neutrophils challenged by nonopsonized wild type Helicobacter pylori strain J99 (J99 wt), and its isogenic mutants with deletions of the SabA adhesin (J99/SabA-) and HPNAP (J99/NAP-)...

Produces a wide variety of effects, including t cell proliferation, differentiation, metabolism, expression of cell surface adhesins, recruit migrating inflammatory cells, and t release of other inflammatory mediators Stimulates proliferation and differentiation of macrophages, neutrophils, and mast cells... 

Recruits and f proliferation of inflammatory cells and progenitor cells, stimulates production of acute phase proteins, f growth and differentiation of keratinocytes Potent chemotactic activity, f expression of cell surface adhesins, stimulates the release of repair enzymes Promotes growth, differentiation, and activation of neutrophils and macrophages... 

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