Sialic acid binding

Figure 5.24 Space-filling model (green) of the sialic acid binding domain of hemagglutinin with a bound inhibitor (red) Illustrating the different binding grooves. The sialic acid moiety of the Inhibitor binds in the central groove. A large hydrophobic substituent, Ri, at the Cz position of sialic acid binds in a hydrophobic channel that runs from the central groove to the bottom of the domain. (Adapted from S.J. Watowich et al.. Structure 2 719-731, 1994.)...

S. A. Kalovidouris, O. Blixt, A. Nelson, S. Vidal, W. B. Turnbull, J. C. Paulson, and J. F. Stoddart, Chemically defined sialoside scaffolds for investigation of multivalent interactions with sialic acid binding proteins, /. Org. Chem., 68 (2003) 8485-8493. 

Burmeister, W. P., Guilligay, D., Cusack, S., Wadell, G., and Amberg, N. (2004). Crystal structure of species D adenovirus fiber knobs and their sialic acid binding sites. J. Virol. 78, 7727-7736. 

Influenza virus particles are spheroidal and approximately 100 nm in diameter. The outer-membrane envelope contains 500 copies of hemagglutinin (HA) trimers and 100 copies of neuraminidase tetramers. The hemagglutinin constitutes the receptor sites for a-sialoside ligands. X-ray analyses show that the three sialic acid binding pockets reside 46 A apart, each trimer being separated on the virion surface by about 65-110 A [42],... 

Reichert A, Nagy JO, Spevak W, Charych D (1995) Polydiacetylene liposomes functionalized with sialic-acid bind and colorimetrically detect influenza-virus. J Am Chem Soc 117 829-830... 

Unemo, M., Aspholm-Hurtig, M., liver, D., Bergstrom, J., Boren, T., Danielsson, D., and Teneberg, S. (2005). The sialic acid binding SabA adhesin of Helicobacter pylori is essential for nonopsonic activation of human neutrophils. J. Biol. Chem. 280, 15390-15397. 

Ratanapo S, Ngamjunyaporn W, Chulavatnatol M. Sialic acid binding lectins from leaf of mulberry (Moms alba). Plant Science 1998 139 141-148. 

The Indian horse-shoe crab Carcinoscorpius rotunda caudu also contains a sialic acid-binding lectin (carcinoscorpin), which was purified almost to homogeneity, and had205 a molecular weight of 420,000. 

Kaludov, N., Brown, K. E., Walters, R. W., Zabner, J. and Chiorini, J. A. (2001). Adeno-associated virus serotype 4 (AAV4) and AAV5 both require sialic acid binding for hemagglutination and efficient transduction but differ in sialic acid linkage specificity. J. Virol. 75, 6884-6893. 

Tang, S., Shen, Y. J., DeBellard, M. E., Mukhopadhyay, G., Salzer,J. L., Crocker, P. R., and Filbin, M. T. (1997). Myelin-associated glycoprotein interacts vdth neurons via a sialic acid binding site at ARG118 and a distinct neurite inhibition site./ Cell Biol. 138, 1355-1366. 

When the high-resolution structure of influenza virus N9 neuraminidase (NA) was determined, it was noted that the conserved residues involved in sialic acid binding were located in a crevasse [17]. Residues within this deep depression are conserved whereas the residues about the rim vary with serotype. This suggests that conserved residues are hidden from antibody recognition. The authors suggested that such architecture might... 

Heerze, L D, Smith, R H, Wang, N, Armstrong, G D, Utilization of sialic acid-binding synthetic peptide sequences derived from pertussis toxin as novel anti-inflammatory agents, Glycobiology, 5, 427-433, 1995. 

Fig. (3). Uie HAl subunits of each HA molecule form the head of protein that contains sialic acids binding sites, top left, and antibodies binding sites, top right (reproduced from Nature with the permission of McMillan Publi iers).

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