Nanoparticle-based biosensors immunosensors

Particularly attractive for numerous bioanalytical applications are colloidal metal (e.g., gold) and semiconductor quantum dot nanoparticles. The conductivity and catalytic properties of such systems have been employed for developing electrochemical gas sensors, electrochemical sensors based on molecular- or polymer-functionalized nanoparticle sensing interfaces, and for the construction of different biosensors including enzyme-based electrodes, immunosensors, and DNA sensors. Advances in the application of molecular and biomolecular functionalized metal, semiconductor, and magnetic particles for electroanalytical and bio-electroanalytical applications have been reviewed by Katz et al. [142]. 

This book provides a comprehensive compilation of seven chapters, with important contributions of several authors. Chapter 1 reviews the recent research in using nanoparticle labels and multiplexed detection in protein immunosensors. This chapter summarizes recent progress in development of ultrasensitive electrochemical devices to measure cancer biomarker proteins, with emphasis on the use of nanoparticles and nanostmctured sensors aimed for use in clinical cancer diagnostics. Based on recent strategies focused on nanomaterials for electrochemical biosensors development. Chap. 2 discusses the development of new... 

Monoamine oxidase amperometric biosensor based on SPE were also modified with MWCNT by using the drop casting technique for the determination of antidepressants in model solutions and dosage forms. The authors used BSA protein which provided a matrix for the immobilization of the enzyme and protection of the enzyme activity when glutaraldehyde is used as a linker. Serafin et aZ. developed a label free dual immunosensor for the determination of human growth and prolactin hormones. The electrochemical immunosensor was based on CNT modify carbon SPE platform with the presence of poly(ethylene-dioxythiophene) (PEDOT) and gold nanoparticles. Again, the hybrid nano-material composite facilitated a proper immobilization of the antibody on the electrode matrix. 

Kim DJ, et al. Organic electrochemical transistor based immunosensor for prostate specific antigen (PSA) detection using gold nanoparticles for signal amplification. Biosensors Bioelectronics 2010 25 2477-82. http //dx.doi.Org/10.1016/j.bios.2010.04.013. 

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