MUME

Nanda, S., Kato, Y. and Asano, Y., A new (i )-hydroxy-nitrile lyase from Prunus mume asymmetric synthesis of cyanohydrins. Tetrahedron, 2005, 61, 10908. 

A New (/ )-Hydroxynitrile Lyase from Prunus mume for Asymmetric Synthesis of Cyanohydrins... 

A new hydroxynitrile lyase (HNL) was isolated from the seed of Japanese apricot Prunus mume). It accepts benzaldehyde and a large number of unnatural substrates for the addition of HCN to produce the corresponding (7 )-cyanohydrins in excellent optical and chemical yields. A new high-performance liquid chromatography (HPLC)-based enantioselective assay technique was developed for the enzyme, which promotes the addition of KCN to benzaldehyde in a buffered solution (pH 4.0). Asymmetric synthesis of (7 )-cyanohydrins by a new HNL is described (Figure 8.4). ... 

Procedure 1 Activity Measurement and Partial Purification of HNL from P. mume... 

Ripened Lfme fruits P. mume) (obtained from local fruit market in June and stored at 4 °C until use, 1 kg or more) potassium cyanide benzaldehyde R)- and (5)-mandelonitrile (NH4)2S04 citrate buffer (pH 4.0) potassium phosphate buffer (pH 6.0) hexane isopropanol... 

Ripened Ume fruit (P. mume) was taken and the fleshy cover was removed to obtain the seeds. The upper layer of the seeds was cracked with a hammer to give the soft kernels inside. Those kernels were collected and crushed in the process homogenizer at 4 °C, with 10 mM potassium phosphate buffer, pH 6.0, to give a milky suspension. The suspension was filtered through four layers of cheesecloth to remove the insoluble part. The HNL activity shown by P. mume extract was 6.9 U mg in the milky suspension. ... 

Carbonyl compounds (5 g) were taken in appropriate solvent (DIPE, TBME or DBE, 50 mL) saturated with 5 mL of citrate buffer (pH 4.0). A solution of partially purified (/f)-HNL from kernels of P. mume (50 U mmol of substrate) followed by acetone-cyanohydrin (1.5 equiv) was added to the reaction mixture. 

We have found a new (/ )-hydroxynitrile lyase from Japanese apricot (P. mume). The new enzyme accepts a broad array of substrates, ranging from aromatic, heteroaromatic, bicyclic to aliphatic carbonyl compounds, and yields the corresponding cyanohydrins with excellent enantioselection. 

Rhamnosyl(l 6)(2"-acetylglucoside) Prunus mume flowers Rosaceae 346... 

Yoshikawa, M. et al.. New flavonol oligoglycosides and polyacylated sucroses with inhibitory effects on aldose reductase and platelet aggregation from flowers of Prunus mume, J. Nat. Prod., 65, 1151, 2002. 

Prunus mume (Sieb.) Sieb. et Zucc. Wu Mai (Black plum) (fruit) Prudomenin, malic acid, succinic acid, citric acid, tartaric acid, amygdalin.33-53 Treat biliary ascariasis and hookworm. 

Chuda, Y., H. Ono, M. Ohnishi-Kameyama, K. Matsumoto, T. Nagata, and Y. Kikuchi. 1999. Mumefural, citric acid derivative improving blood fluidity from fruit-juice concentrate of Japanese apricot (Prunus mume Sieb. et Zucc.). J. Agric. Food Chem. 47 828-831. 

Fang, T. T., and J. H. Huang. 1999. Extraction, fractionation and identification of bitter oligopeptides and amino acids in mei fruit (Prunus mume Sieb. et Zucc.). J. Beijing Forestry Univ. 21 61-71. 

Armeniaca ansu, A. mandsharica, A. sibirica, A. vulgaris, Eriobotrya japonica, Prunus mume, P. domestica, P. glandulosa, P. japonica, P. padus, P. armeniaca, Pyrrosia adnascens Hordeum vulgare Myristica fragrans... 

L. sativa, Macrocarpium officinalis, Matricaria chamomilla, Oxalis corriculaza, O. corymbosa, Prunus mume, P. persica, Ribes mandshurica, Viburnum sargenti, Vitis amurensis, V. vinifera Mallotus repandus... 

Angelica polymorpha, A. sinensis, Geranium dahuricum, G. eriostemon, G. sibiricum, G. wlassowianum, G. wilfordi, Maytenus diversifolia, M. confertiflorus, Prunus mume, Sarcandra glabra, Typhonium giganteum... 

Artemisia vulgarts, Camellia sinensis, Prunus mume Oxyria digyna Centella asiatica... 

Camellia sinensis, Crocus sativus, Prunus mume Melochia tomentosa Campanula rotundifolia, C. palustris Hedera helix... 

Anagalis arvensis, Cucurbita maxima Curcuma longa, Santalum album Curcuma aromatica Quercus robur Prunus mume Jatropha gospiifolia... 

Eleutherocossus senticosus, Gelidium cartilagineum, Laminaria digitata, L. saccharine, L. longicruris, Lobelia pulmonaria, Panax ginseng, P. quinquefolium, Phragmites australis, Prunus mume, Urtica urens Populus tremuloides... 

Aleurites moluceanu, Artemisia vulgarts, Camellia sinensis, Cannabis sativa, Daucus carota, Hibiscus rosa-sinensis, H. sabdariffa, Lycoperiscon esculentum, Prunus mume, Zea mays Tagetes patula... 

Wu Mei (Mume fructus), Wu Bei Zi (Chinensis galla), Ying Su Ke (Papaveris somniferi pericarpium), Qian Shi (Euryalis semen), Rou Dou Kou (Myristicae semen), He Zi (Chebulae fructus), Chi Shi Zhi (Halloysitum rubrum), Lian Zi (Nelumbinis semen), Fu Long Gan (Terra flava usta) and Jin Ying Zi (Rosae laevigatae fructus)... 

Zhi Gan Cao (Glycyrrhizae radix preparata) 5 g Sheng Jiang (Zingiberis rhizoma recens) 3 g Wu Mei (Mume fructus) 1 piece... 

Aliphatic aldehydes have been converted to their (R)-cyanohydrins using a bipha-sic system to accommodate hydroxynitrile lyase enzyme (from the Japanese apricot, Prunus mume) as the enantioselective catalyst.251... 

Mizuma, T., et al. 2005. Intestinal SGLT1-mediated absorption and metabolism of benzyl beta-glucoside contained in Prunus mume Carrier-mediated transport increases intestinal availability. Biochim Biophys Acta 1722 218. 

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