MRC-5 cells

Xylan-based microparticles were also evaluated regarding their in vitro toxicity. In fact, cross-liked (CLM) and spray-dried microparticles (SDM) based on xylan and ESIOO were produced in order to carry UA and avoid its side effects, namely hepatotoxicity and nephrotoxicity. Additionally, CLM and SDM dispersions at concentrations of 50, 125, 250, and 500 pg/ml were placed in contact with human embiyonic Ixmg fibroblasts (MRC-5 cells)... 

Fig. 14. Viability of MRC-5 cells after incubation for 24h with spray-dried (SDM) and cross-linked xylan microparticles (CLM) containing UA.

ORDINARY DIFFERENTIAL EQUATION MODELS 17.3.1 Contact Inhibition in Microcarrier Cultures of MRC-5 Cells... 

However, the nucleoside analogue 164 was found to be devoid of activity against HSV-1, HSV-2, VZV and the cytomegalovirus (CMV) in human fibroblast (MRC-5) cells. In this case the decreased conformational flexibility resulting from the introduction of the cyclopropyl group into 164 appeared to be unfavourable for interaction with the enzymes involved (vide supra. Sect. 2.9) [222]. Likewise, the cyclopropylpyrimidine 166c-f and 167, the cyclopropyl-purine nucleosides 168 showed no antiviral activity against HSV-1, HSV-2, HCMV and HIV-1 in cell culture, Eq. (66) [223]. 

Continuous cell lines not derived from humans or primates and well-characterized diploid human cells lines with a finite lifespan, for example MRC-5 cells, are considered low risk cell lines. Poorly characterized mammalian cell lines are classified as medium risk cells. Among high risk cell lines, are human and primate tissue cells, cell lines carrying endogenous pathogens, and cell lines manipulated after experimental infections. 

The MRC-5 cell line, derived in 1966 from normal human lung tissue (Jacobs et al., 1970), is adherent and shows a fibroblast morphology. These cells are well known owing to their susceptibility to several virus types, being employed in assays related to viral transfection, in cytotoxicity evaluation, and in vaccine production. 

Figure 3.5.1 Growth of MRC-5 cells on experimental surfaces with varying chemistries.

CMV stock is prepared by infecting confluent MRC-5 cells in plastic flasks at 37°C. Check by microscopy for CPE. At 50-75% CPE, decant medium, add fetal calf serum to 20%, and freeze at -70°C. After freezing, prepare tenfold... 

Passage MRC-5 cells into 24-well plates (1 3 split) and allow to grow to 90% confluency. 

Cell-free VZV can be prepared from MRC-5 cells as for Mewo cells, with the of probe sonication for 3 x 15 s (with 15-s rests intervening) after scraping. Virus from MRC-5 cells may be more stable if preinfection incubation is required. 

For VZV, Mewo cells give clear plaques, which are much easier to read than those obtained with MRC-5 cells as used by some workers. 

Garcinia livingstonei T. Anderson (root bark) en/-Naringeninyl-(I-3 a,II-8)-II-4 -0-methylnaringenin (171). (+)-volkensiflavone (154) (+)-morelloflavone (159). Antiparasitic activity against Plasmodium falciparum, Leishmania infantum, Trypanosoma brucei brucei, and T. cruzi, and cytotoxicity against MRC-5 cells. Mbwambo et al., 2006[85]. 

Cyclic 3, 5 -phosphates of ara-A (7, B = adenyl) [12], ara-C (7, B = cytosyl) [15] and thioinosine (8, R = H) have been synthesized [16]. The acylated thioinosine derivative (8, R = Me(CH2)i4CO, ECso 6 //M) has been shown to readily enter S49 mouse lymphoma cells and then be metabolized to thioinosine 5 -monophosphate catalyzed by phosphodiesterase and deacylation. More recently, the cyclic phosphonate analogue (9) of the antiviral (5)-l-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (HPMPC) has been prepared and shown to have comparable activity against HSV-2, both in vitro in MA-104 and MRC-5 cells and in vivo in a mouse encephalitis model, to that of the parent drug [17]. The cyclic phosphonate (9) was converted to HPMPC inside the cells, before phosphorylation to the active diphosphate. 

Cytotoxic against hepatocellular carcinoma (Hep G2, Hep 3B), breast carcinoma (MDA-MB-231, MCF-7), lung carcinoma (A-549), and embryonic lung (MRC-5) cell lines [73]... 

Normal fetal fibroblast (MRC-5) cells, 3480 Normal-phase high performance liquid... 

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